Title: The expression of interleukin 23(IL-23) in model of asthmatic mice
Abstract: Objective:To study the expression of IL-23 in model of asthma in asthmatic mice.Methods:Mouse models were established and divided into two groups,normal control group and asthma group.The levels of IL-23 in serum were detected by ELISA,the levels of IL-23p19mRNA and IL-23p19 protein in lung tissue were detected respectively by RT-PCR and immunohistochemistry.Spleen T lymphocytes were isolated and cultivated in vitro.The levels of IL-23p19mRNA and IL-23p19 protein in T lymphocytes were detected respectively by RT-PCR and Western blot.Results:Compare to normal control group,the level of IL-23 in serum was higher in asthma group(P0.01),the levels of IL-23p19mRNA and IL-23p19 protein in lung tissues were higher in asthma group(P0.01),and the levels of IL-23p19mRNA and IL-23p19 protein in spleen T lymphocytes was higher in asthma group(P0.01).Conclusion:IL-23 may involve in the pathogenesis of asthma,and may have influence on T lymphocytes.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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