Title: Effect of hypothermia on the immunoreactivity of microtubule associated protein 2 during cerebral ischemia/reperfusion
Abstract: Objective To investigate the effect of hypothermia on the immunoreactivity of microtubule associated protein 2 (MAP2) during cerebral ischemia/reperfusion. Methods The cerebral ischemia models of gerbils were achieved by occluding bilateral carotid arteries, and the insult time was 10 min. The gerbils were randomly divided into sham-operation group, normothermia reperfusion group and hypothermia reperfusion group. The normothermia and hypothermia reperfusion groups were further divided into subgroups according to the reperfusion time (6 h, 48 h and 96 h). The immunoreactivity of MAP2 was determined by using immunohistochemical staining and computer image analysis system. The survival neurons in hippocampal CA1 were counted 48 h and 96 h after reperfusion. Results The immunoreactivity of MAP2 in hippocampal CA1 6 h, 48 h and 96 h after reperfusion in normothermia reperfusion group were decreased to 81%, 69% and 51% that in sham-operation group (P0.01). The immunoreactivities of MAP2 in hippocampal CA1 in hypothermia reperfusion group were 93%, 86% and 71% that in sham-operation group, much higher than that in normothermia reperfusion group (P0.05 or P0.01). The number of survival neurons in hippocampal CA1 96 h after reperfusion in normothermia reperfusion group was only 5% that in sham-operation group (P0.01), while the number of survival neurons 96 h after reperfusion in hypothermia reperfusion group was 47%, much more than that in normothermia reperfusion group (P0.01). Conclusion Hypothemia could attenuate neuronal damage through inhibition of MAP2 degradation during cerebral ischemia/reperfusion.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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