Title: Protective effect of resveratrol on human umbilical vein endothelial cell injury induced by hydrogen peroxide
Abstract: AIM To investigate the protective effect of resveratrol(Res) on the injury of human umbilical vein endothelial cell(HUVECs) induced by hydrogen peroxide(H2O2) in vitro and to explore its mechanism.METHODS The HUVECs was subcultured in vitro and used for experiment.They were divided into five groups as follows: control group,H2O2-injured group(300 μmoL/L),low-dosage of Res group(5 μmoL/L),mid-dosage of Res group(10 μmoL/L) and high-dosage of Res group(20 μmoL/L).MTT was used to determine the HUVECs viability.The contents of malondialdehyde(MDA),lactate dehydrogenase(LDH),superoxide dismutase(SOD),reduced glutathion(GSH) were measured by the commercial kits.Apoptosis rate of the HUVECs was analyzed by flow cytometry.The expression of apoptosis-related protein caspase-3 was measured by western blot.RESULTS Compared with the control group,H2O2 significantly decreased HUVECs viability,increased the contents of MDA,LDH,and decreased the contents of SOD and GSH,and meantime increased the apoptosis rate and caspase-3 expression in H2O2-injured group.CONCLUSION Res can protect HUVECs from HUVECs from H2O2-induced injury through lowering the expression of caspase-3,and inhibiting the endothelial cel apoptosis.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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