Title: Determination of aflatoxin M1 and B1 in food by ultra- high performance liquid chromatography tandem mass spectrometry and solid phase extraction
Abstract: The method for detecting aflatoxin M1( AFM1) and aflatoxin B1( AFB1) from foods by solid- phase extraction and UPLC- MS /MS is established. The samples are extracted with acetonitrile and purified SPE column,then dried under nitrogen,and finally constant volume with 50% methanol water solution. Ultra performance liquid chromatography- tandem mass spectrometry( UPLC- MS /MS) determination is experimented by the mode of electrospray positive ionization( ESI+) and multiple reaction monitoring( MRM),and take methanol and 0. 1%( v/v) formic acid aqueous solution as mobile phase,the flow rate is0. 4 ml /min,take ZORBAX Eclipse XDB- C18 chromatographic column( 100 mm × 3. 0 mm × 1. 8 μm) as analytical column. AFM1 has a good linear relationship in the range of 0. 1 ~ 50ug /kg and 0. 1 ~ 10ug /kg for AFB1. The correlation coefficient are above 0. 999 both,and the recovery rate is 83. 75% ~ 97. 8%. The detection limit is 0. 05 μg /kg for AFM1,0. 02 μg /kg for AFB1. The analytical method is fast,accurate detection,sensitivity and recovery advantages,suitable for food aflatoxin M1 and B1 detection.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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