Title: Determination of Benzoquanmine in Liquid Products of Milk by LC-MS / MS
Abstract: A method was developed for the determination of benzoquanmine in liquid products of milk by high performance liquid chromatography-tandem mass spectrometry( HPLC-MS / MS). The conditions of extraction and parameters of LC-MS were optimized. The sample was enriched and purified with an LC-C18SPE cartridge,then separated on a ZORBAX Eclipse Plus C18column using a mixture of acetonitrile-water( 15 ∶ 85) as mobile phase at a flow rate of 0. 3 mL / min. The detection of benzoquanmine was performed by mass spectrometry( ESI-MS) under positive-ion electrospray ionization mode. The two precursor-product ion pairs,m / z 104. 1 and m / z 146. 0,were used as the quantitative and qualificative ions, respectively. The external standard method was used for quantitative analysis. The results showed that a good linearity for benzoquanmine existed in the range of 2-100 μg / L with a correlation coefficient of 0. 999 1. The limits of detection( LODs) and limits of quantitation( LOQs) were 1. 0 μg / kg and 3. 0 μg / kg,respectively. The average recoveries of benzoquanmine at three spiked levels were in the range of 77%-105%,with relative standard deviations( RSDs) of 2. 3%-5. 0%. The proposed method was simple and accurate,and was suitable for the determination of benzoquanmine in liquid products of milk.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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