Title: Determination of baicalin and tanshinone II A in Chidan pills by RP-HPLC
Abstract: Objective To establish an RP-HPLC method for simultaneous determination of the contents of baicalin and tanshinoneⅡA in Chidan(traditional Chinese medicines) pills.Methods The column of Agilent Zorbax Extend C18(4.6 mm×250 mm,5 μm) was used,the mobile phase was consisted of methanol,water adjusted to pH 3 with phosphoric acid and acetonitrile,gradient elution was performed;the detection wave-length was set at 270 nm and the column temperature was 25 ℃.Results The linear range of baicalin and tanshinoneⅡA were 15.80-126.40 mg·L-1 and 5.04-80.56 mg·L-1 respectively.The average recoveries of baicalin and tanshinoneⅡA were 99.0% with RSD of 1.28%,98.3% with RSD of 1.12%(n=5),respectively.Conclusions The method is simple,sensitive and good reproducibility.It can be used for quality control of Chidan pills.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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