Title: Production and identification of monoclonal antibodies to chCD25
Abstract: BALB/c mice were immunized with purified recombinant chCD25.Spleen lymphotes were fused with SP2/0 myeloma cells.Seven hybridoma cell lines(1E1,1G1,4H5,5E1,6A1,6C2,6C9)secreting monoclonal antibodies(McAb)were produced by three-time limited dilution method.The titers of ascetic fluids of the seven McAbs were up to 106 in indirect ELISA.Besides 1E1 belonging to IgG2b,κ,isotypes and subclasses of other McAbs belonged to IgG1,κ.The results of western bolt and immunocytochemistry showed that these McAbs can specifically react with recombinant chCD25 and EGFP-chCD25,separately.It is proved that all these McAbs provides a useful instrument to study the expression of chCD25 in vivo and vitro in future.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot