Title: Antitumor effect and mechanism of pseudolarix acid B on various human tumor cells
Abstract: Objective To evaluate the antitumor effect of pseudolarix acid B(PLAB) on various human tumor cell lines and its mechanism in vitro.Methods MGC803,AGS,SMMC7721,LOVO,A375,SK-28 and 624 mel in logarithmic growth phase were intervented with different concentrations of PLAB.The survival rate of tumor cells were tested by MTT;The expression of peroxisome proliferator activated receptorγ(PPARγ)mRNA was detected by RT-PCR;Cell cycle was analyzed by flow cytometry.Results The survival rate of tumor cells decreased significantly after the intervene of PLAB in a dose and time-dependent manner(all P0.05).The expression of PPARγ mRNA was the strongest in MGC803 cells,the secondary in SMMC7721 cells,the lowest in LOVO cells.After the treatment of PLAB(10 μmol/L),the cell percentage of AGS,MGC803 and SK-28 in the G2/M phase increased obviously.Conclusions PLAB can significantly induce G2 / M phase arrest on tumor cells in vitro,which may be related to the inhibition of p53 expression in the tumor cells expressing wild-type p53,and be related to the activation of PPARγ expression in the tumor cells expressing mutant p53.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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