Title: Determination of Chlorogenic Acid in Mulberry Leave by TLC-UV Spectrophotometry
Abstract: Objective To establish a TLC-UV spectrophotometry method to determine chlorogenic acid in Mulberry Leaves.MethodsThe chlorogenic acid was extracted by methanol and was separated with TLC.The contents of chlorogenic acid was determined at 329 nm with UV spectrophotometry method.ResultsThere was a good linear between mass of chlorogenic acid and absorbance within the range of 50~250 μg(r=0.999 6).The average recovery was 95.0% and RSD=1.2%.ConclusionThe method was simple sensitive and accurate.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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