Title: Injury of Rat Cardiomyocytes Exposed to Nonionizing Radiation and Its Mechanism
Abstract: Objective To study the injury of rat cardiomyocytes exposed to nonionizing radiation and its mechanism. Methods Primarily cultured cardiomyocytes were irradiated by 9 GHz 950 mW/cm2 microwave pulse (MWP)and 0~100 MHz 6×104 V/m electromagnetic pulse (EMP)for 72 h respectively ,then a series of apparatus including atom force microscope,laser scanning confocal microscope and flow cytometer were used to detect the changes of cell membrane conformation,structure and function. Results Slower pulsation,abnormal conformation,lower viability ,the significantly increased percentage of apoptosis and necrosis were observed in cardiomyocytes after irradiation by MWP and EMP respectively (P0.01?雪. Formation of holes differenting in number and in size induced by cytomembrane electroporation were found in cytomembrane of cardiomyocytes exposed to MWP and EMP. The concentration of Na+?K+?Ca2+?Cl-?Mg2+ and P3+ increased significantly (P0.01)in cell culture medium. Conclusion Cardiomyocytes were susceptibly injured by nonionizing radiation. Pulse electromagnetic field could induce cardiomyocytes electroporation,and resulted in abnormal cell conformation,structure and function . Electroporation was one of the most pivotal mechanisms for the non-thermal effects induced by electromagnetic radiation.The changes of cytomembrane permeability and structure induced by electromagnetic radiation revealed certain regularity.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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