Title: Tissue Culture Technique of Euonymus bungeanus
Abstract: [Objective] The purpose of the research was to promote the rapid propagation of Euonymus bungeanus seedlings and keep their good traits.[Method] With the immature stems and leaves of E.bungeanus as materials,they were inoculated on MS media with different hormone levels to generate seedlings by induction after sterilization.[Result] The medium MS + 6-BA 0.3 mg/L + NAA 0.10 mg/L was the optimum medium to generate auxiliary buds by induction from E.bungeanus explants.The seedlings could be induced from E.bungeanus leaves successfully on the medium MS+6-BA 0.3 mg/L+NAA 0.10 mg/L + 2,4-D 0.05 mg/L and the induced seedlings had brightly green leaves and grew vigorously.On the medium MS+6-BA 0.3 mg/L+NAA 0.10 mg/L,the differentiation coefficient of seedlings was highest and the seedlings grew best.On the medium 1/2 MS + IBA 0.4 mg/L+NAA 0.10 mg/L+phloridzin 3 mg/L,the rooting rate of seedlings was higher and their roots were more developed.On the substrate peat+vermiculite+perlite(5:3:2),the rooting time of transplanted seedlings was shortest,being 10 d and their rooting rate and survival rate were highest,being 95.6% and 85.48% resp.[Conclusion] The research supplied technical support for the tissue culture and raising seedling of E.bungeanus.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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