Title: Cloning and Primary Analysis of the Function of GhFTL1 Gene in Cotton(Gossypium hirsutum L.)
Abstract: A FT-like homolog was isolated from Gossypium hirsutum L.CV.Xinluzao 33 through RT-PCR and RACE,which was named GhFTL1 gene(accession number: HM631972).The 525-bp open reading frame encoded a putative protein of 174 amino acids with high similarity to FT proteins of different plants which contains two critical residues and conserved motif,both of which identified as diagnostic of true FT.GhFTL1 gene showed constitutive expression in root,stem,flower,leaf,fiber,and ovule.The levels of transcript in leaf and ovule were higher than that in other tissues.Phylogenetic analysis at the amino acid level confirmed that the isolated sequence is FT-like,and also showed that GhFTL1 had shorter genetic distance with MdFT1 compared with other 18 FT homologs confirmed to inducing early flowering from 13 different plants.Over-expression of GhFTL1 in Arabidopsis using the CaMV 35S promoter resulted in significant accelerated flowering compared with wild-type plants.Based on our results,we concluded that GhFTL1 was responsible for inducing flowering and may be one of important components for the flowering pathway in cotton.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 2
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