Title: Simultaneous determination of six flavonoids in Scutellariae Radix by MEKC-DAD
Abstract: Objective: To establish a method for simultaneous determination of baicalin,baicalein,wogonoside, wogonin,oroxylin A and apigenin in Scutellariae Radix of different batches and its processed products by micellar electrokinetic chromatography with diode array detection(MEKC-DAD). Methods: The buffer solution(pH 8. 4) of 40 mmol·L-1 sodium hydrogen phosphate-15 mmol·L-1 sodium borate-40 mmol·L-1 SDS-15% acetonitrile-7. 5% propyl alcohol was used as the electrophoretic medium and the standard uncoated fused silica capillary(75 μm × 64. 5 cm,56 cm of effective length) was used as the separation pathway. The separation voltage was 20 kV with a detection wavelength of 280 nm; the column temperature was maintained at 25 ℃,and the sample was injected at 5 kPa,6 s. Results: The abovementioned six flavonoids showed good linearity(r 0. 9990) in the range of the tested concentration,and the average recoveries of the method were between 96. 20%-103. 6%. Conclusion: The method is simple,accurate and reproducible,and can be used for quality control and evaluation of Scutellariae Radix.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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