Title: Analyzing the mutations of rpsL gene in Mycobacterium tuberculosis clinical isolates by PCR-RFLP
Abstract:Objective To observe the mutations of rpsL gene in M.tuberculosis streptomycin-resistant isolates,and to develop a new method for detecting drug resistance.Method Analyzing the mutations of rpsL gene ...Objective To observe the mutations of rpsL gene in M.tuberculosis streptomycin-resistant isolates,and to develop a new method for detecting drug resistance.Method Analyzing the mutations of rpsL gene codon 43 in M.tuberculosis clinical isolates with PCR-RFLP.Results M.tuberculosis strain H 37 R V was used as a control.The rpsL gene fragments from 13 drug-sensitive strains could be digested by restriction endonuclease MboⅡ.31(83.8%)of 37 streptomycin resistant strains were not restricted by MboⅡ.2 of 12 non-streptomycin-resistant strains hadn't also sites of MboⅡ.Conclusions Most M.tuberculosis streptomycin-resistant strains could be observed the mutations situated at codon 43 of genes encoding the ribosomal S12 protein (rpsL).PCR-RFLP might become a simple,rapid and reliable means of detecting drug resistance in clinical isolates.Read More
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot