Title: Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC
Abstract: Objective: To establish a HPLC method for determination of three constituents in Qingfei Yihuo Pills.Method: Baicalin,geniposide and genipin-1-O-β-gentiobioside were determined simultaneously by HPLC on a agilent Zorbax SB-C18 column with the methanol(A)-1.0% formic acid anhydrous(B)-acetonitrile(C) in gradient elution as the mobile phrase.The detection wavelength was set at 238,440 nm and the flow rate was 1.0 mL.min-1.Result: The standard curves for baicalin,geniposide and genipin-1-O-β-gentiobioside were linear within the range of 0.165-1.650,0.106-1.060,0.035-0.350 μg(r 0.999 7),and the average recoveries were over 96.75%(RSD 2.05%).Conclusion: The method is simple and accurate and could be used for quality control of Qingfei Yihuo Pills.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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