Title: Apoptosis of smooth muscle cells induced by radiation
Abstract:Objective To study the effects and mechanism of 188 Re on apoptosis of cultured smooth muscle cells (SMCs), and to explore the value of radiation induced SMCs apoptosis for preventing restenosis. Meth...Objective To study the effects and mechanism of 188 Re on apoptosis of cultured smooth muscle cells (SMCs), and to explore the value of radiation induced SMCs apoptosis for preventing restenosis. Methods The SMCs cultured in vitro were irradiated by 188 Re with different doses. The trypan blue exclusion test, flow cytometry, JAM test, transmission electron microscopy and immunocytochemistry assay were used to investigate the effects of β particles on apoptosis of SMCs, such as cell viability, cell apoptosis rate, DNA fragmentation, cell ultrastructural changes and related gene expression. Results There were no significant changes of SMCs viability, cell apoptosis rate, DNA fragmentation and cellular ultrastructure in low dose irradiation group, compared with control group. High dose radiation (2.96 GBq/L) on SMCs showed that viable cell proportion was markedly decreased, while cell apoptosis rate and DNA fragmentation were significantly increased, and cellular ultrastructure was destroyed. The expression of p53, bax gene was up regulated and bcl 2/bax was decreased while SMCs apoptosis occurred. Conclusions Low dose radiation on SMCs, which could inhibit completely SMCs proliferation, didn't show any effects on cell viability, cell apoptosis rate, cell ultrastructure and DNA fragmentation. High dose radiation could result in significant SMCs apoptosis. Up regulated p53, bcl 2 and bax gene took a part in cell apoptosis induced by radiation. Low dose and low dose rate radiation appeared to be an ideal intravascular radiotherapy for preventing restenosis, which could not only inhibit SMCs proliferation, but also preserve cell viability and integrity.Read More
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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