Title: Immobilization of Cellulase of Lentinula edodes in Sodium Alginate-gelatin
Abstract: Cellulase of Lentinula edodes was immobilized on sodium alginate-gelatin carried with glutaraldehyde.The impact of the concentration of sodium alginate,gelatin concentration,amount of calcium chloride concentration,amount of the cellulase,amount of glutaraldehyde concentration and cross linking time on immobilization were analyzed.The thermostability,the optimum temperature and pH value were studied.The results showed that the optimal concentration of sodium alginate and gelatin were 3.5% and 3.0% respectively and the concentration of glutaraldehyde was 1.0%.In comparision with the free enzyme,the optimal pH of the immobilized cellulase shifted 0.4 to acidity and the optimum reaction temperature had increased by 5℃.The storage stability of the immobilized enzyme was obviously increased compared to that of the free one.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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