Title: Effect and mechanism of homocysteine on dopaminergic neurons
Abstract: Objective To study the effects and mechanism of homocysteine (Hcy) on dopamine neuron in mice model with parkinson’s disease (PD) induced by 6-hydroxydopamine (6-OHDA) in vivo.Methods 40 rats were divided into 4 groups. 6 OHDA or solvent of 6 OHDA was focally administrated to induce PD, 2 hours later Hcy or 0.9% Sodium Chloride were administrated in the ipsolateral substantia nigra. Behavioral testing, immunohistochemical technique biochemistry technique were used to detect the injury of substantia. Results The rotary turns of PD rats induced by 6-OHDA showed significantly increasing after treatment with Hcy compared with the controls ( P 0 05). Also the numbers of TH-stained neurons were decreased, and dendrites were fragmented and truncated. Free radicals were increased and antioxidant enzyme decreased. Conclusions Focal infusion of Hcy into the substantia nigra increased the vulnerability of the dopaminergic neurons to 6-OHDA-induced degeneration, it seemed likely that the endangering effect of Hcy is due to exacerbating oxidative stress.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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