Title: Sequencing analysis of cellulase gene cel9A and localization of gene cel48A from bacillus licheniformis GXN151
Abstract:Three types of clones expressing the carboxymethylcellulase activities were isolated from the genomic library of Bacillus licheniformis GXN151 by the substrate-based screening method. The DNA sequenci...Three types of clones expressing the carboxymethylcellulase activities were isolated from the genomic library of Bacillus licheniformis GXN151 by the substrate-based screening method. The DNA sequencing indicated the clone pGXNP11 harbors the cellu-lase gene ce19A and partial sequence of cel48A. The cel9A gene was 1899 bp, encoding family 9 endoglycohydrolase with 633 amino acids. The N-terminal amino acid 21~456 of Cel9A formed family 9 glycosyl hydrolase catalytic domain. Amino acid 480~565 was family 3 carbohydrate binding modules. The cel9A gene was found in an operon together with a partial cistron designated cel48A which encodes a product highly similar to bacterial exocellobiohydrolases belonging to family 48 of the glycosyl hydrolases. The cel48A gene was located on the 4 kb EcoRI fragment or 10 kb SalI fragment by the method of chromosome walking.Read More
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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