Title: EFFECT OF EXTRACTS FROM VEGETABLE POWDER AND β-CAROTENE ON PROLIFERATION AND APOPTOSIS OF HUMAN LUNG CANCER CELLS
Abstract: Objective: To observe the effects of fat soluble extracts from vegetable powder (FEVP) and β carotene on proliferation and apoptosis of cultured YTMLC 90 human lung cancer cells. Methods: The proliferation was evaluated by MTT test. The induction of apoptosis was evaluated by morphological change, DNA fragmentation analysis, DNA content analysis combined with flow cytometric analysis. Results: FEVP was found to inhibit cell proliferation in 2.5×10 -6 mol/L,2.5×10 -7 mol/L, 5×10 -8 mol/L concentration and β carotene was found to inhibit cell proliferation in 1×10 -4 mol/L and 1×10 -5 mol/L concentration. Higher concentration of FEVP and β carotene could inhibit nuclear mitosis and induce morphologic changes consistent with apoptosis in YTMLC 90 cancer cells such as cellular shrinkage, chromatin condensation and nuclear fragmentation, DNA fragmentation ladder, the presence of a sub G 1 apoptotic peak. Conclusion: FEVP inhibits the growth of YTMLC 90 probably via the induction of apoptosis. There is dose dependent relation. Moreover, the effect of FEVP is stronger than that of β carotene.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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