Title: Genetic Diversity Analysis of Superior Ornamental Germplasms from Liquidambar formosana
Abstract:ISSR marker technology was used to analyze the genetic diversity between 12 ornamental germplasm samples.The optimized ISSR-PCR amplification system was created with the orthogonal method,and it was a...ISSR marker technology was used to analyze the genetic diversity between 12 ornamental germplasm samples.The optimized ISSR-PCR amplification system was created with the orthogonal method,and it was as follows: 20 μL reaction system containing 2μL of PCR buffer,Mg2+ with 1.5 mmolL-1,dNTP with 0.15mmol/L,primers with 0.4μmo L-1,and 80 ng of DNA template.The result showed that 131 bands were applied with 19 ISSR primers,of which 94 were polymorphic and the polymorphism strip ratio was 71.75%.The effective alleles,genetic diversity and Shannon information index were 1.482,0.3012 and 0.4674 respectively,which reflected the high level of genetic diversity among 12 samples.Further clustering analysis indicated these 12 germplasm samples were divided into 3 groups,among which the No.4 and No.11 samples had the highest genetic similarity.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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