Title: Genetic Diversity of Dendrobium Species (Orchidaceae) Plants in China by ISSR
Abstract:Objective To probe the relationship among Dendrobium species(Orchidaceae).Methods 14 primers with good polymorphism screened from 55 ISSR primers were used to conduct ISSR analysis and dimorphic data ...Objective To probe the relationship among Dendrobium species(Orchidaceae).Methods 14 primers with good polymorphism screened from 55 ISSR primers were used to conduct ISSR analysis and dimorphic data of DNA fragments were used to construct the dendrogram by UPGMA.Results Totally 123 bands were amplified and 112 of them were polymorphic accounting for 91.06%.The average number of DNA bands amplified by each primer was 8.79.Nei's gene diversity(H),Shannon's information index(I) and effective number of alleles(NE) were 0.351 9,0.528 2 and 1.594 5,respectively.The genetic similarity among all the tested clonal cultivars ranged from 0.090 9 to 0.695 7,averaging 0.377 9,which indicated that the genetic basis was relatively rich.Conclusion Clustering results revealed that the dendrogram constructed with ISSR molecular makers was identical with the classic taxonomic system.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 2
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