Title: Preparation of Standard Baicalin and Determination by SPE-HPLC
Abstract: A preparation of standard baicalin was described.Pure baicalin was obtained by extraction with ethanol under ultrasonic,precipitated by acid and then purified by steps.Determining of baicalin by HPLC from Scutcllaria baicalensis and several Chinese herbal medicines was also reported.According to the difference of undetermined samples,different pretreatment were used.Waters Plus-C_(18) SPE column was used for pre-separation,methanol-water-ice acetic acid was used as mobile phase and baicalin was separated and determined.The recovery was 92-103%,with RSD of 1.4~2.2%.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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