Title: The Apoptosis-inducing Effect of TRAIL and Low-concentration 5-Fu on Colon Cancer Cells
Abstract: Objective This study aims to detect whether TRAIL can synergize with 5 Fu to kill colon cancer cell line HT29,and its possible mechanism.Methods HT29 cells were cultured in the regular condition.MTT(microculture tetrazolium dye) was used to evaluate the cytotoxic effects at 24 hours after TRAIL and 5 Fu,alone or combined,were added to cells.Flow Cytometry Assay was also used to test the apoptosis proportion among these cells.Electron microscopy was used to observe the subcellular morphologic change.Results ①HT29 cells were not sensitive to TRAIL.The rate of killing was 4.50%±0.26% with TRAIL concenlzation of 100 ng/ml.And ID 501 000 μg/ml;The cells were comparatively more responsive to 5 Fu with an apparent dose effect relationship.This cytotoxicity was mainly due to cell apoptosis.②The combination of TRAIL and 5 Fu presented synergistic effect on HT29 cells.Subtoxic concentration of TRAIL(100 ng/ml) together with subtoxic concentration of 5 Fu(100 μg/ml),could kill 60.00% ±0.45% of HT29 cells.③The cytotoxicity was mainly attributed to cell apoptosis as proved by flow cytometry assay and electron microscopy.Conclusion TRAIL can synergize with subtoxic 5 Fu to effectively kill colon cancer cell line HT29 even these cells are resistant to TRAIL alone.Apoptosis is the main mechanism of this killing effect.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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