Title: Effect of pretreatment with NBP on brain edema and blood brain barrier in rats during cerebral ischemia reperfusion injury
Abstract: Objective To observe the effects of 3-n-butylphthalide( NBP) pretreatment on brain edema and blood brain barrier( BBB) in rats with cerebral ischemia reperfusion injury( CIRI). Methods Ninety male SD rats were randomly divided into sham operation group( Sham group),Model group( IR group),low dose group of NBP( NBPⅠgroup),medium dose group of NBP( NBP Ⅱ group) and high dose group of NBP( NBP Ⅲ group),18 rats in each group. The model of middle cerebral artery occlusion were subjected by suture method. The degree of brain edema was analyzed through dry /wet weight measurement,the permeability of BBB was detected by collecting extravascular Evans blue( EB) in the brain cortex.The changes of MMP-9 and TIMP-1 mRNA expression were assessed by Real-Time Quantitative PCR. Results After NBP pretreatment on CIRI rats,content of water and EB were decreased obviously,the expression of MMP-9 was reduced significantly,the expression of TIMP-1 was greatly increased( P 0. 01),the difference between NBP Ⅱ group and NBP Ⅲ group was not obvious. Conclusion The pretreatment of NBP has preventive protective effect against cerebral ischemia reperfusion injury in rats,which may be related to regulate the expression of MMP-9 /TIMP-1 and reduce the permeability of BBB and the degree of brain edema.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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