Title: Construction of the eukaryotic expression plasmid compatible with the detection system of shuttle plasmid pZ189
Abstract: In order to separate two kinds of plasmids existing in the same mammalian cell simultaneously in a certain experiment system, inserting inactivation was used to reconstruct eukaryotic cell expression plasmids and change their antibiotic resistance. Using pREP9 that contains ampicillin resistance and pMAMneo that contains ampicillin and kanamycin resistance as template, we got two new eukaryotic cell expression plasmids: pREP9 amp - and pMAMneo amp - which can be selected in E.coli by kanamycin instead of ampicillin. pREP9 amp - can be used for consistent expression while the latter one directs dexamethasone inducible expression. These vectors can be isolated from other plasmids through antibiotic resistant selection conveniently when the cells were transfected with two kinds of plasmids and compatible with the mutation detection system of shuttle plasmid pZ189.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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