Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2058124388', 'doi': 'https://doi.org/10.1016/j.plasmid.2008.01.004', 'title': 'New pSC101-derivative cloning vectors with elevated copy numbers', 'display_name': 'New pSC101-derivative cloning vectors with elevated copy numbers', 'publication_year': 2008, 'publication_date': '2008-05-01', 'ids': {'openalex': 'https://openalex.org/W2058124388', 'doi': 'https://doi.org/10.1016/j.plasmid.2008.01.004', 'mag': '2058124388', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/18295880', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/2435062'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/j.plasmid.2008.01.004', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S160206373', 'display_name': 'Plasmid', 'issn_l': '0147-619X', 'issn': ['0147-619X', '1095-9890'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://europepmc.org/articles/pmc2435062?pdf=render', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5035133050', 'display_name': 'James M. Peterson', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I173911158', 'display_name': 'Iowa State University', 'ror': 'https://ror.org/04rswrd78', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I173911158']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'James Peterson', 'raw_affiliation_strings': ['Department of Veterinary Microbiology, Veterinary Medical Research Institute, Iowa State University, 1802 University Boulevard, Building #6, Ames, IA 50011, USA'], 'affiliations': [{'raw_affiliation_string': 'Department of Veterinary Microbiology, Veterinary Medical Research Institute, Iowa State University, 1802 University Boulevard, Building #6, Ames, IA 50011, USA', 'institution_ids': ['https://openalex.org/I173911158']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5000215433', 'display_name': 'Gregory J. Phillips', 'orcid': 'https://orcid.org/0000-0002-0048-512X'}, 'institutions': [{'id': 'https://openalex.org/I173911158', 'display_name': 'Iowa State University', 'ror': 'https://ror.org/04rswrd78', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I173911158']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Gregory J. Phillips', 'raw_affiliation_strings': ['Department of Veterinary Microbiology, Veterinary Medical Research Institute, Iowa State University, 1802 University Boulevard, Building #6, Ames, IA 50011, USA'], 'affiliations': [{'raw_affiliation_string': 'Department of Veterinary Microbiology, Veterinary Medical Research Institute, Iowa State University, 1802 University Boulevard, Building #6, Ames, IA 50011, USA', 'institution_ids': ['https://openalex.org/I173911158']}]}], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2850, 'currency': 'USD', 'value_usd': 2850, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 0.985, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 45, 'citation_normalized_percentile': {'value': 0.835908, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 93, 'max': 94}, 'biblio': {'volume': '59', 'issue': '3', 'first_page': '193', 'last_page': '201'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10123', 'display_name': 'Molecular Mechanisms of DNA Damage Response', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10123', 'display_name': 'Molecular Mechanisms of DNA Damage Response', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10120', 'display_name': 'Bacterial Physiology and Genetics', 'score': 0.9991, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10521', 'display_name': 'Ribosome Structure and Translation Mechanisms', 'score': 0.9975, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/cole1', 'display_name': 'ColE1', 'score': 0.88133097}, {'id': 'https://openalex.org/keywords/replicon', 'display_name': 'Replicon', 'score': 0.745203}, {'id': 'https://openalex.org/keywords/t-dna-binary-system', 'display_name': 'T-DNA Binary system', 'score': 0.6239767}, {'id': 'https://openalex.org/keywords/secondary-structure-prediction', 'display_name': 'Secondary Structure Prediction', 'score': 0.453394}], 'concepts': [{'id': 'https://openalex.org/C2777014509', 'wikidata': 'https://www.wikidata.org/wiki/Q5141800', 'display_name': 'ColE1', 'level': 4, 'score': 0.88133097}, {'id': 'https://openalex.org/C22744801', 'wikidata': 'https://www.wikidata.org/wiki/Q172778', 'display_name': 'Plasmid', 'level': 3, 'score': 0.82401377}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.8040008}, {'id': 'https://openalex.org/C18103114', 'wikidata': 'https://www.wikidata.org/wiki/Q1720369', 'display_name': 'Replicon', 'level': 4, 'score': 0.745203}, {'id': 'https://openalex.org/C80343103', 'wikidata': 'https://www.wikidata.org/wiki/Q7667796', 'display_name': 'T-DNA Binary system', 'level': 5, 'score': 0.6239767}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.50317496}, {'id': 'https://openalex.org/C58337764', 'wikidata': 'https://www.wikidata.org/wiki/Q976651', 'display_name': 'Origin of replication', 'level': 4, 'score': 0.50020075}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.46691445}, {'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.44224998}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.32221496}, {'id': 'https://openalex.org/C92087593', 'wikidata': 'https://www.wikidata.org/wiki/Q427389', 'display_name': 'Vector (molecular biology)', 'level': 4, 'score': 0.23957375}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.09639332}], 'mesh': [{'descriptor_ui': 'D015964', 'descriptor_name': 'Gene Expression Regulation, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D005821', 'descriptor_name': 'Genetic Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D005822', 'descriptor_name': 'Genetic Vectors', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D009154', 'descriptor_name': 'Mutation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D000667', 'descriptor_name': 'Ampicillin', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000667', 'descriptor_name': 'Ampicillin', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D002701', 'descriptor_name': 'Chloramphenicol', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002701', 'descriptor_name': 'Chloramphenicol', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D003001', 'descriptor_name': 'Cloning, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004265', 'descriptor_name': 'DNA Helicases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004265', 'descriptor_name': 'DNA Helicases', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D004268', 'descriptor_name': 'DNA-Binding Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004268', 'descriptor_name': 'DNA-Binding Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D029968', 'descriptor_name': 'Escherichia coli Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D029968', 'descriptor_name': 'Escherichia coli Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D005822', 'descriptor_name': 'Genetic Vectors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005838', 'descriptor_name': 'Genotype', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007612', 'descriptor_name': 'Kanamycin', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007612', 'descriptor_name': 'Kanamycin', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010957', 'descriptor_name': 'Plasmids', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010957', 'descriptor_name': 'Plasmids', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D012097', 'descriptor_name': 'Repressor Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012097', 'descriptor_name': 'Repressor Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D015534', 'descriptor_name': 'Trans-Activators', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015534', 'descriptor_name': 'Trans-Activators', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}], 'locations_count': 4, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/j.plasmid.2008.01.004', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S160206373', 'display_name': 'Plasmid', 'issn_l': '0147-619X', 'issn': ['0147-619X', '1095-9890'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc2435062', 'pdf_url': 'https://europepmc.org/articles/pmc2435062?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'acceptedVersion', 'is_accepted': True, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435062', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'acceptedVersion', 'is_accepted': True, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/18295880', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc2435062', 'pdf_url': 'https://europepmc.org/articles/pmc2435062?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'acceptedVersion', 'is_accepted': True, 'is_published': False}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 37, 'referenced_works': ['https://openalex.org/W11602674', 'https://openalex.org/W1185556181', 'https://openalex.org/W1503130701', 'https://openalex.org/W1922509375', 'https://openalex.org/W1963905705', 'https://openalex.org/W1966999414', 'https://openalex.org/W1980223784', 'https://openalex.org/W1981497491', 'https://openalex.org/W1994503800', 'https://openalex.org/W1995924881', 'https://openalex.org/W1996066959', 'https://openalex.org/W1999075212', 'https://openalex.org/W2003371514', 'https://openalex.org/W2008467306', 'https://openalex.org/W2009314432', 'https://openalex.org/W2019877703', 'https://openalex.org/W2023617715', 'https://openalex.org/W2036330256', 'https://openalex.org/W2045991415', 'https://openalex.org/W2054941036', 'https://openalex.org/W2055044163', 'https://openalex.org/W2061051066', 'https://openalex.org/W2077033339', 'https://openalex.org/W2077441699', 'https://openalex.org/W2080274676', 'https://openalex.org/W2081884876', 'https://openalex.org/W2086148694', 'https://openalex.org/W2092944982', 'https://openalex.org/W2093738748', 'https://openalex.org/W2141653906', 'https://openalex.org/W2144427468', 'https://openalex.org/W2160425393', 'https://openalex.org/W2164253505', 'https://openalex.org/W2166744717', 'https://openalex.org/W27699049', 'https://openalex.org/W4242893999', 'https://openalex.org/W51065063'], 'related_works': ['https://openalex.org/W2589658700', 'https://openalex.org/W2525157896', 'https://openalex.org/W2472136834', 'https://openalex.org/W2262337278', 'https://openalex.org/W2169925970', 'https://openalex.org/W2136360997', 'https://openalex.org/W2113692303', 'https://openalex.org/W2048333445', 'https://openalex.org/W2004045188', 'https://openalex.org/W1999857608'], 'abstract_inverted_index': {'Mutations': [0], 'that': [1, 29], 'increase': [2], 'the': [3, 7, 43, 55, 58, 80, 125, 129, 158, 163], 'copy': [4, 39], 'number': [5], 'of': [6, 15, 20, 46, 75, 82, 122, 128, 137, 142, 157], 'pSC101': [8, 59], 'replicon': [9], 'have': [10], 'been': [11], 'used': [12, 113], 'for': [13, 99, 154], 'construction': [14], 'new': [16, 159], 'cloning': [17, 70], 'vectors.': [18], 'Replacement': [19], 'glutamate': [21], 'at': [22, 31], 'position': [23], '93': [24], 'in': [25, 67, 114], 'RepA': [26], 'yields': [27], 'plasmids': [28, 94], 'replicate': [30], 'medium': [32], '(27': [33], 'copies/cell)': [34, 38], 'and': [35, 88, 102, 108], 'high': [36], '(∼240': [37], 'numbers.': [40], 'Based': [41], 'on': [42], 'crystal': [44], 'structure': [45], 'RepE,': [47], 'a': [48, 115, 146], 'structurally': [49], 'similar': [50], 'replication': [51], 'initiator': [52], 'protein': [53, 126], 'from': [54, 145], 'F': [56], 'factor,': [57], 'repA': [60], 'mutants': [61, 121], 'are': [62], 'predicted': [63], 'to': [64, 105, 118], 'be': [65], 'defective': [66], 'dimerization.': [68], 'The': [69, 93, 110], 'vectors': [71, 111, 160], 'permit': [72], 'increased': [73], 'expression': [74, 141], 'gene': [76], 'products': [77], 'along': [78], 'with': [79, 90], 'advantages': [81], 'pSC101-derivative': [83, 149], 'plasmids,': [84], 'including': [85], 'stable': [86], 'maintenance': [87], 'compatibility': [89], 'ColE1': [91], 'plasmids.': [92], 'also': [95], 'allow': [96], 'blue/white': [97], 'screening': [98], 'DNA': [100], 'inserts': [101], 'impart': [103], 'resistance': [104], 'ampicillin,': [106], 'chloramphenicol': [107], 'kanamycin.': [109], 'were': [112], 'genetic': [116], 'assay': [117], 'suppress': [119, 162], 'temperature-sensitive': [120, 164], 'ffh,': [123], 'encoding': [124], 'component': [127], 'Escherichia': [130], 'coli': [131], 'signal': [132], 'recognition': [133], 'particle,': [134], 'by': [135], 'overproduction': [136], '4.5S': [138, 143], 'RNA.': [139], 'While': [140], 'RNA': [144], 'wild': [147], 'type': [148], 'plasmid': [150], 'was': [151], 'not': [152], 'sufficient': [153], 'suppression,': [155], 'use': [156], 'did': [161], 'phenotype.': [165]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2058124388', 'counts_by_year': [{'year': 2024, 'cited_by_count': 5}, {'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 5}, {'year': 2019, 'cited_by_count': 5}, {'year': 2018, 'cited_by_count': 4}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 3}, {'year': 2012, 'cited_by_count': 5}], 'updated_date': '2024-09-10T11:17:31.796255', 'created_date': '2016-06-24'}