Title: Expression of 14-3-3zeta in stage T1 non-small cell lung cancer and its relationship with clinical pathological significance
Abstract: Objective To investigate the expression of 14-3-3ζ and the clinical significance in stage T1 nonsmall cell lung cancer(NSCLC). Methods Specimens of NSCLC and adjacent normal lung tissues were collected from 110 patients. Immunohistochemistry, Western blotting and RT-PCR analysis were used to detect the expression of 14-3-3ζ in stage T1 NSCLC and normal lung tissues. Results The results showed that the overexpression of 14-3-3ζ mRNA and protein was observed in stage T1 NSCLC tissues than in normal lung cancer tissue, with not any correlation with age and gender and histological types, but with significant correlation with histological grades and lymph node metastasis. The expression of 14-3-3ζ increased, the lower the differentiation of NSCLC was, the expression of 14-3-3ζ was higher in the positive lymph node metastasis cancer tissues than in the negative cases. The survival curves analysis showed that 14-3-3ζ expression was closely related to the prognosis of patients. Conclusion The overexpression of 14-3-3ζ is related to histological grades, lymph node metastasis and poor clinical outcome, which indicates that 14-3-3ζ might be used as a developmental and prognostic biomarker for NSCLC.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot