Title: Construction of NP Gene Retrovirus Vector for Avine Influenza Virus and Identification of Recombinant
Abstract: According to the theory of antisense RNA,we inserted full-long the cDNA of NP gene and 5' and 235bp of AIV H14N5 into retroviral vector pLXSN, named pLXas-NP and pLXas-5 '-NP respectively. The retroviral plasmid were transfected into packaging cell line PA317 with lipofectin, then by G418 colony screening method. Firstly the virus RNAs(vRNA)were extracted from the virus-containing super- natant of packaging cell lines, detected the vRNA by Nested-PCR. Secondly infect NIH3T3 with the virus-containing supernatant. Screened the high-titer clones.The recombinant retroviruses were obtained.The way is a rapidly and simply preparation for latter research.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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