Title: An efficient plasmid-driven system for the generation of influenza virus-like particles for vaccine
Abstract: We established an efficient plasmid-driven system for the generation of infectious influenza VLPs containing a virus-like RNA segment entirely from cDNAs. To generate influenza VLPs, we used the RNA polymerase I system for the intracellular synthesis of influenza virus RNAs. Human embryonic kidney cells (293T) were transfected with plasmids encoding the influenza A virus structural proteins and with a plasmid encoding an influenza virus-like viral RNA (vRNA), which contained an antisense copy of the cDNA for green fluorescence protein (GFP) flanked by an RNA polymerase I promoter and terminator (pPolI-GFP). Influenza virus-like particles containing GFP vRNA that were infectious and expressed GFP in infected cells were generated. We also generated VLPs lacking the NS gene by eliminating the plasmid for the NS RNA segment from the set of plasmids required for infectious influenza virus production. Because of its efficiency, this system would be useful in studies of influenza virus replication and particle formation as well as for production of vaccines.
Publication Year: 2001
Publication Date: 2001-10-01
Language: en
Type: article
Indexed In: ['crossref']
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