Title: Study on Interaction between Plasmid pBR322DNA and Endonuclease EcoRI
Abstract:The interaction of cleaving and binding between DNA and endonucleases EcoRI was observed by atomic force microscope(AFM).The findings showed that in the presence of Mg2+ in buffer,endonucleases EcoRI ...The interaction of cleaving and binding between DNA and endonucleases EcoRI was observed by atomic force microscope(AFM).The findings showed that in the presence of Mg2+ in buffer,endonucleases EcoRI can cleave pBR322DNA on one specific recognition site;while,if Ca2+ took the place of Mg2+ in buffer,endonucleases EcoRI can bind pBR322DNA on the specific recognition site.The cleaving and binding ratio would increase with the increase of concentration of endonucleases.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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