Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1973754742', 'doi': 'https://doi.org/10.1016/0378-1119(94)00863-n', 'title': 'Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants', 'display_name': 'Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants', 'publication_year': 1995, 'publication_date': '1995-05-01', 'ids': {'openalex': 'https://openalex.org/W1973754742', 'doi': 'https://doi.org/10.1016/0378-1119(94)00863-n', 'mag': '1973754742', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/7607511'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/0378-1119(94)00863-n', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S75702506', 'display_name': 'Gene', 'issn_l': '0378-1119', 'issn': ['0378-1119', '1879-0038'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5000838820', 'display_name': 'Humberto Flores', 'orcid': 'https://orcid.org/0000-0003-1598-0569'}, 'institutions': [], 'countries': ['MX'], 'is_corresponding': False, 'raw_author_name': 'Humberto Flores', 'raw_affiliation_strings': ['Department of Molecular Biology, Instituto de Biotecnologia/UNAM, Cuernavaca, Mor. 62271, México'], 'affiliations': [{'raw_affiliation_string': 'Department of Molecular Biology, Instituto de Biotecnologia/UNAM, Cuernavaca, Mor. 62271, México', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5043445804', 'display_name': 'Joel Osuna', 'orcid': 'https://orcid.org/0000-0002-8904-0724'}, 'institutions': [], 'countries': ['MX'], 'is_corresponding': False, 'raw_author_name': 'Joel Osuna', 'raw_affiliation_strings': ['Department of Molecular Biology, Instituto de Biotecnologia/UNAM, Cuernavaca, Mor. 62271, México'], 'affiliations': [{'raw_affiliation_string': 'Department of Molecular Biology, Instituto de Biotecnologia/UNAM, Cuernavaca, Mor. 62271, México', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5052159588', 'display_name': 'Joseph Heitman', 'orcid': 'https://orcid.org/0000-0001-6369-5995'}, 'institutions': [{'id': 'https://openalex.org/I4210126298', 'display_name': 'Duke Medical Center', 'ror': 'https://ror.org/03njmea73', 'country_code': 'US', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210126298', 'https://openalex.org/I4210144876']}, {'id': 'https://openalex.org/I4210153043', 'display_name': 'Duke University Hospital', 'ror': 'https://ror.org/04bct7p84', 'country_code': 'US', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210126298', 'https://openalex.org/I4210144876', 'https://openalex.org/I4210153043']}, {'id': 'https://openalex.org/I1344073410', 'display_name': 'Howard Hughes Medical Institute', 'ror': 'https://ror.org/006w34k90', 'country_code': 'US', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I1344073410']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Joseph Heitman', 'raw_affiliation_strings': ['Departments of Genetics and Pharmacology, and the Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC, 27710, USA'], 'affiliations': [{'raw_affiliation_string': 'Departments of Genetics and Pharmacology, and the Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC, 27710, USA', 'institution_ids': ['https://openalex.org/I4210126298', 'https://openalex.org/I4210153043', 'https://openalex.org/I1344073410']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5061749338', 'display_name': 'Xavier Soberón', 'orcid': 'https://orcid.org/0000-0002-4498-5628'}, 'institutions': [], 'countries': ['MX'], 'is_corresponding': False, 'raw_author_name': 'Xavier Soberón', 'raw_affiliation_strings': ['Department of Molecular Biology, Instituto de Biotecnologia/UNAM, Cuernavaca, Mor. 62271, México'], 'affiliations': [{'raw_affiliation_string': 'Department of Molecular Biology, Instituto de Biotecnologia/UNAM, Cuernavaca, Mor. 62271, México', 'institution_ids': []}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 3, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2940, 'currency': 'USD', 'value_usd': 2940, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 1.166, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 28, 'citation_normalized_percentile': {'value': 0.653478, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 87, 'max': 88}, 'biblio': {'volume': '157', 'issue': '1-2', 'first_page': '295', 'last_page': '301'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10878', 'display_name': 'CRISPR and Genetic Engineering', 'score': 0.9994, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10878', 'display_name': 'CRISPR and Genetic Engineering', 'score': 0.9994, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10207', 'display_name': 'Advanced biosensing and bioanalysis techniques', 'score': 0.9988, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10432', 'display_name': 'DNA and Nucleic Acid Chemistry', 'score': 0.9983, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/saturated-mutagenesis', 'display_name': 'Saturated mutagenesis', 'score': 0.699117}, {'id': 'https://openalex.org/keywords/site-directed-mutagenesis', 'display_name': 'Site-directed mutagenesis', 'score': 0.5430017}, {'id': 'https://openalex.org/keywords/recognition-sequence', 'display_name': 'Recognition sequence', 'score': 0.5162925}], 'concepts': [{'id': 'https://openalex.org/C130123407', 'wikidata': 'https://www.wikidata.org/wiki/Q417754', 'display_name': 'EcoRI', 'level': 4, 'score': 0.9736196}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.75677735}, {'id': 'https://openalex.org/C143065580', 'wikidata': 'https://www.wikidata.org/wiki/Q3285695', 'display_name': 'Mutant', 'level': 3, 'score': 0.7450683}, {'id': 'https://openalex.org/C109129352', 'wikidata': 'https://www.wikidata.org/wiki/Q7426583', 'display_name': 'Saturated mutagenesis', 'level': 4, 'score': 0.699117}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.6022256}, {'id': 'https://openalex.org/C16318435', 'wikidata': 'https://www.wikidata.org/wiki/Q149299', 'display_name': 'Mutagenesis', 'level': 4, 'score': 0.58683825}, {'id': 'https://openalex.org/C103408237', 'wikidata': 'https://www.wikidata.org/wiki/Q2642668', 'display_name': 'Site-directed mutagenesis', 'level': 4, 'score': 0.5430017}, {'id': 'https://openalex.org/C100594029', 'wikidata': 'https://www.wikidata.org/wiki/Q7302658', 'display_name': 'Recognition sequence', 'level': 4, 'score': 0.5162925}, {'id': 'https://openalex.org/C128319531', 'wikidata': 'https://www.wikidata.org/wiki/Q219715', 'display_name': 'Restriction enzyme', 'level': 3, 'score': 0.49324626}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.45071778}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.40828943}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.35574675}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.23596561}], 'mesh': [{'descriptor_ui': 'D004247', 'descriptor_name': 'DNA', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D015246', 'descriptor_name': 'Deoxyribonuclease EcoRI', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D015246', 'descriptor_name': 'Deoxyribonuclease EcoRI', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D006639', 'descriptor_name': 'Histidine', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D017354', 'descriptor_name': 'Point Mutation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D011487', 'descriptor_name': 'Protein Conformation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D000595', 'descriptor_name': 'Amino Acid Sequence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001483', 'descriptor_name': 'Base Sequence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001665', 'descriptor_name': 'Binding Sites', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004247', 'descriptor_name': 'DNA', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004247', 'descriptor_name': 'DNA', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D017931', 'descriptor_name': 'DNA Primers', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015246', 'descriptor_name': 'Deoxyribonuclease EcoRI', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015246', 'descriptor_name': 'Deoxyribonuclease EcoRI', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D007700', 'descriptor_name': 'Kinetics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008958', 'descriptor_name': 'Models, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008969', 'descriptor_name': 'Molecular Sequence Data', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016133', 'descriptor_name': 'Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D015183', 'descriptor_name': 'Restriction Mapping', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013379', 'descriptor_name': 'Substrate Specificity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/0378-1119(94)00863-n', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S75702506', 'display_name': 'Gene', 'issn_l': '0378-1119', 'issn': ['0378-1119', '1879-0038'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/7607511', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 33, 'referenced_works': ['https://openalex.org/W100656390', 'https://openalex.org/W1514587303', 'https://openalex.org/W1550573517', 'https://openalex.org/W1564791373', 'https://openalex.org/W1567835962', 'https://openalex.org/W1965887510', 'https://openalex.org/W1970657288', 'https://openalex.org/W1970668642', 'https://openalex.org/W1971374540', 'https://openalex.org/W1974081085', 'https://openalex.org/W1978870223', 'https://openalex.org/W1981929180', 'https://openalex.org/W1982744052', 'https://openalex.org/W1982878977', 'https://openalex.org/W1986586522', 'https://openalex.org/W1994212633', 'https://openalex.org/W2006869216', 'https://openalex.org/W2007862184', 'https://openalex.org/W2028622989', 'https://openalex.org/W2033942855', 'https://openalex.org/W2045606892', 'https://openalex.org/W2047034905', 'https://openalex.org/W2049934487', 'https://openalex.org/W2074040142', 'https://openalex.org/W2091745929', 'https://openalex.org/W2092171870', 'https://openalex.org/W2092837927', 'https://openalex.org/W2095489470', 'https://openalex.org/W2133335149', 'https://openalex.org/W2136716105', 'https://openalex.org/W2152845055', 'https://openalex.org/W2911680748', 'https://openalex.org/W83140495'], 'related_works': ['https://openalex.org/W4213263878', 'https://openalex.org/W3140343155', 'https://openalex.org/W2790413672', 'https://openalex.org/W2413723606', 'https://openalex.org/W2410954609', 'https://openalex.org/W2407313553', 'https://openalex.org/W2373469313', 'https://openalex.org/W2038274100', 'https://openalex.org/W1973754742', 'https://openalex.org/W131204990'], 'abstract_inverted_index': None, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1973754742', 'counts_by_year': [{'year': 2014, 'cited_by_count': 2}], 'updated_date': '2024-12-16T03:28:49.105348', 'created_date': '2016-06-24'}