Title: Myroides gitamensis Sp. Nov., L-Asparaginase Producing Bacteria Isolated From Slaughter House Soil Sample in Visakhapatnam, India
Abstract: A novel strain designated as BSH-3 T was isolated from the slaughter house soil sample in Visakhapatnam, India.Cells of this strain are Gram-negative, aerobic, yellowish, rod shaped, non-motile and non-spore forming bacteria.This isolate was observed to grow optimally at 30°C and pH 7.0.It requires 1-6% (w/v) NaCl for its growth.Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strain BSH-3 T belongs to the genus Myroides and is closely related to Myroides odoratimimus (98.4 %).However, DNA-DNA hybridization with M. odoratimimus JCM 7460 T showed a relatedness of 48.2% with respect to strain BSH-3 T .The major cellular fatty acids identified were iso-C 15:0 and iso-C 17:0 3-OH and summed feature 3 (comprising iso-C 15:0 2OH and/or C 16:1 ω7c).The polar lipids of the strain BSH-3 T were phospholipids, aminolipids and phosphatidyl ethanolamine.The G + C content of the genomic DNA was determined to be 33.8 mol%.The enzyme production was carried out by submerged fermentation and the maximum enzyme activity was 85.7 IU showed by 24 hrs BSH-3 T culture at 37°C.Based on the morphological, phenotypic characteristics, phylogenetic inference and chemotaxonomic studies, strain BSH-3 T is proposed as a novel species Myroides gitamensis sp.nov. in the genus Myroides.