Title: Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
Abstract: Biofilms 2003 37°C for 18 hours on an orbital shaker at 200 rpm. After formation of biofilm, the 96-pin lid was placed into a micro-titre tray containing dilutions of tetra-sodium EDTA(0, 5, 10, 15 and 20 mg/ml) and incubated at 37°C for 24 hours. At time intervals of 1, 3, 6 and 24 hours, triplicate pins were removed, for each concentration of tetrasodium EDTA, washed by inversion in 5ml in phosphate buffered saline (PBS) and then sonicated in a sonicating water bath, for 15 minutes in 3ml PBS. Triplicate log dilutions of the biofilm suspension were then plated onto cysteine lactose electrolyte deficient (CLED) agar plates by automated spiral-plater, and incubated for 24 hours at 37°C, before performing automated colony counts. The MBEC was designated as, the lowest concentration at which growth was reduced by at least 99% when compared to the control. Results: Initial biofilm viable cell count levels averaged log 6 cfu/peg/ml. Of twelve Gram-positive bacteria tested, nine, including Staphylococcus aureus (3), methicillin resistant Staphylococcus aureus (3), Coagulase negative staphylococcus (CNS)(2), and Enterococcus sp. (1), had an MBEC of <5 mg/ml. The remaining three Grampositive bacteria were CNS with MBEC of 20-40 mg/ml. In comparison, of the twelve Gram-negative bacteria tested, seven, including Klebisella sp. (3), Enterobacter cloacae (1), Proteus sp. (2), E. coli (1), had an MBEC of <5 mg/mL. The remaining five including Enterobacter cloacae (2), Stenotrophomonas maltophilia (1), Pseudomonas aeruginosa (1) and Acinetobacter baumanii (1), had MBEC values of 10-20mg/mL. Conclusion: The MBEC of all the organisms tested, when using the LMCD, were <40 mg/ml tetra-sodium EDTA.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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