Title: Effects of microRNA-17-5p inhibitors on proliferation and apoptosis of human osteosarcoma cell line SOSP-9607.
Abstract:Objective:To determine the effects of microRNA-17-5p inhibitors on proliferation and apoptosis of human osteosarcoma cell line SOSP-9607.Methods:MTT assay was used to examine the inhibitive percentage...Objective:To determine the effects of microRNA-17-5p inhibitors on proliferation and apoptosis of human osteosarcoma cell line SOSP-9607.Methods:MTT assay was used to examine the inhibitive percentage of hsa-miR-17-5p inhibitors on the proliferation of SOSP-9607 cells.Cultured SOSP-9607 cells were divided into two groups:control group and experimental group.The control group contained two groups:the negtive control and the normal control.In experimental group,hsa-miR-17-5p inhibitors were used to inhibit the activity of miR-17-5p.It contained three groups with different doses.The apoptosis and the cell cycle were 1measured by flow cytometry.Cultured SOSP-9607 cells were also divided into two groups:control group and experimental group.Results:Hsa-miR-17-5p inhibitors inhibited SOSP-9607 cell proliferation in a dose-dependent manner(P0.01),while the control group had no obvious effects on cell proliferation(P0.05).With the increment of concentration of inhibitors from 50 nmol/L to 200 nmol/L,the inhibition percentage increased significantly (P0.01).The apoptosis ratio of experimental group([9.6±1.8)%] was obviously higher than the apoptosis ratio of control group ([3.5±0.4)%](P0.01).Conclusions:The hsa-miR-17-5p inhibitors plays an important role in cell proliferation and apoptosis in SOSP-9607 cells by down-regulated the miR-17-5p.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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