Title: An analysis by ISSR of genetic diversities in Stylosanthes germplasms.
Abstract: Forty-eight Stylosanthes germplasms were studied for an analysis of genetic diversity by ISSR.Fourteen effective primers selected from 68 ISSR primers were used for PCR,and 155 of the 156 DNA fragments amplified,showed polymorphisms.The average binds from each primer was 11.1 and the average percentage of polymorphic bands was 99.36%.The coefficient of genetic conformability among the germplasms was 0.445-0.975.The results from POPGENE analysis indicated that the average information index(I) of Shannon was 0.355 3,gene diversity(H) of Nei was 0.227 9,and effective number of alleles(NE) was 1.388 7.Based on analyses of cluster and principal components,the 48 Stylosanthes germplasms used in this study could be divided into 5 groups: S.hamata,S.capitata,S.scabra,S.seabrana and S.guianensis,in which the genetic differentiation of S.guianensis group was the largest.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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