Title: Detection of enterotoxin genes of Escherichia coli from piglets by multiplex-PCR assay.
Abstract: A method based on the polymerase chain reaction(PCR) was developed to detect heat-stable enterotoxin(STa,STb)genes and heat-labile enterotoxin(LT)gene from enterotoxigenic Escherichia coli(ETEC).The PCR primers were designed according to the conserved sequence of STa,STb and LT genes and the expected sizes of PCR-amplified productions were 182,108 and 336 bp,respectively.This method was used to detect enterohemorrhagic E.coli(EHEC) EDL933,Streptcoccus,Salmonella typhimrium and Pasteurella pneumotropic strain,none was found to contain STa,STb and LT genes homologous sequences,and standard strains of 102 cfu/mL can be detected.Among 11 E.coli strains isolated from piglets with diarrhea which came from Foshan in Guangdong,5 carried the gene for STa homologous sequences,2 possessed the gene for STb,3 carried the gene for LT,2 of which carried simultaneously the genes for STb and LT.The results confirmed that this PCR method show high specificity and sensitivity for rapid detection of enterotoxin genes and epidemiological investigation of E.coli strains.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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