Title: Apparatus and Methodology for Microcarrier Cell Culture
Abstract: Publisher Summary Interest in development of improved apparatus and methodology for Microcarrier (MC) cell culture is strong at this time as a result of recognition of a growing number of useful products for human and animal therapy that can be produced by anchorage-dependent cells (ADCs). This chapter reviews the direction of evolution of apparatus and methods for the special purpose of microcarrier (MC) cell culture, on the basis of pragmatic considerations and the observations. MC cell culture is the method of choice for producing useful products from ADCs, although cell propagators providing large continuous surface areas may have value in certain applications. It is accepted that MC cell culture offers the following principal advantages: a high ratio of growth surface to culture volume is provided; a single large production unit can replace a number of smaller units; mixed, homogeneous MC cell cultures can be sampled, monitored, and controlled more easily than inhomogeneous systems; harvesting of cells and extracellular products can be done easily; and MC cell cultures can be scaled up readily using conventional equipment (fermentors) used for microbial processes. Problems unique to MC cell culture systems are related to the need to manage the initial attachment of cells to MCs as well as to manage the detachment, or harvest of cells from MCs at the end of one stage for inoculation of the next stage. Serious studies of on-line analysis and computerized control of MC cell culture systems have been initiated and rapid advances may be expected.
Publication Year: 1986
Publication Date: 1986-01-01
Language: en
Type: review
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 18
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