Abstract: A human embryonic lung fibroblast(LuMA) cell line was established for production of a live attenuated varicella-zoster virus vaccine. Using this cell line, we have investigated the possibili ty of using microcarrier culture system for producing cell-associated and cell-free varicellazoster virus. We have also investigated the utilization of glucose and amino acids during cell culture and virus propagation in microcarrier culture. It was found that no infectious cell-free viruses appear in the culture medium, and substantial decreases in cell density was observed a cell culture time increase. The cell-free varicella-zoster virus yield in microcarrier culture was much lower than that of the stationary T-flask culture.
Publication Year: 1994
Publication Date: 1994-06-01
Language: en
Type: article
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