Title: Effect of Culture Environment Upon Sporangium and Zoospore Production of Three Species of Phytophthora
Abstract: Various techniques have been used to promote oospore and sporangium production within the genus Phytophthora. For sporangium and zoospore production Gooding and Lucas (2) grew Phytophthora parasitica (Dastur) var. nicotianae (Breda de Haan) Tucker on solid oatmeal agar and stripped aerial mycelium from cultures after 6-20 days growth. The mycelium was then transferred to plates and moistened or floated on water at 24-26°C for 6-10 days. Fresh distilled water was then added to the cultures, they were chilled for 25 min at 8°C, and then returned to room temperature. Zoospores were released after about 15 min. This procedure requires from 16-32 days. Sporangia and zoospores can also be obtained from oatmeal agar cultures by taking small squares of agar with the organism and floating them on water in Petri dishes for 10-12 days, during which time additional mycelium and sporangia develop. The cultures are then chilled for about 30 min and the mycelium and sporangia are then transferred to water at room temperature and zoospores are released. This latter method is also time consuming, and sporangium development and zoospore release are often erratic.
Publication Year: 1969
Publication Date: 1969-01-01
Language: en
Type: article
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Cited By Count: 1
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