Title: Antimicrobial Potentials of Different Solvent Extracts of Justicia landonoides and Plantago lanceolata Against Standard and Drug Resistant Human Bacterial Pathogens
Abstract: Scientists paid attention to search new antimicrobials against multidrug resistant pathogenic microbes' wide therapeutic potentials. The objective of this study was to determine antimicrobial potentials of extracts of Justicia landonoides and Plantago lanceolata against standard and drug resistant human microbial pathogens. Active antimicrobial compounds were extracted using three different solvents (acetone, methanol alnd water). The antimicrobial activities of crude extracts were determined using agar well diffusion assay and broth dilution methods. Penicillin, methicillin alnd amoxicillin were used as positive controls and water as negative control. Minimum inhibitory concentrations and minimum bactericidal concentrations of each crude extracts were also determined against test microorganisms using broth dilution method. Combined effects of crude extracts and commercial antibiotics were also determined. Crude extracts of P. lanceolata showed various degrees of antimicrobial activity towards each standard and drug resistant microbial pathogen with mean zone of inhibition ranges up to 26±3.60 mm against multidrug resistant K. pneumoniae. Of the different crude extracts, acetone extract of J. landanoides showed the highest mean zone of inhibition 55.3 ±3.05mm against E. coli (ATCC2592). Clinical isolate of C. albicans was inhibited by all extracts with mean diameter inhibition zone ranges up to 22±2.64 mm for water extract of P. lanceolata. Specifically, S. aureus (ATCC2923), E. coli (ATCC2592), S. pneumoniae (multidrug resistant), K. pneumoniae (multidrug resistant) alnd S. boydii (ATCC9289) have been shown MIC and MBC ranging from 6.25% to 25% for both plant extracts investigated in this study. The minimum bactericidal concentrations of P. lanceolata against on both Gram positive and Gram negative bacteria were ranges from 12.5 to 25%. According to this study, crude extracts of the plants under investigation have potential inhibitory effects against pathogenic microorganism.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 2
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