Title: Hormonal regulation impact on regeneration of Acacia nilotica L. a nitrogen fixing tree.
Abstract: An efficient regeneration protocol was developed for the establishment of Acacia nilotica L., a nitrogen fixing, multipurpose leguminous tree, through indirect organogenesis. Present investigation was the first attempt to obtained indirect organogenesis in Acacia nilotica L. through cotyledonary node explant of in vitro grown plants. Cotyledonary node (1.0 cm) explant excised from 20 day old in vitro seedling cultured on MS medium supplemented with various concentrations of 2, 4- D alone and in combination with BAP for callus induction. However, hormonal regulation and their concentrations played crucial role in obtaining the callus. Under the optimized conditions, maximum and rapid growth of green callus was obtained after the 25 days of inoculation on MS medium with 2, 4- D (2.0 mg/l) alone or in combination with of 2, 4- D (0.40 mg/l) and BAP (0.20 mg/l ) in combination. Combination of auxin and cytokinin proved to be more effective for callus induction. Shoot induction was also obtained on the same medium after subculturing twice at the time interval of 21 days. The highest number and their elongation of adventitious shoots achieved when activated charcoal (200 mg/l) added on MS medium fortified with 2, 4- D (0.40 mg/l) and BAP (0.2 mg/l). However, the elongated adventitious shoots were rooted on half strength MS medium with IBA (0.5 mg/l) after 20 days. Rooted plantlets were hardened and successfully established in soil with 75% survival rate.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 6
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot