Title: In vitro plantlet regeneration from cotyledonary node explants of Salvadora persica L. a medicinally important desert tree
Abstract: An efficient and reproducible in vitro propagation protocol has been developed for rapid micropropagation of Salvadora persica L. a medicinally and economically important desert tree. The present investigations were carried out with an aim for the development of a tissue culture method for the clonal propagation of this tree. The seeds were germinated on MS half strength medium devoid of growth regulators. The cotyledonary node explants were excised from twenty days old seedlings and inoculated on MS medium supplemented with different concentrations (0.25-2.0 mg l -1 ) of auxins (NAA, IAA and 2,4-D) and cytokinins (BAP & Kinetin) individually as well as in various combinations. The frequency of shoot regeneration from cotyledonary node was affected by various concentrations of auxins, cytokinins and successive transfer of mother explant. Maximum numbers of shoots (17.5±0.02) were obtained on MS medium containing BAP (2.0 mg l -1 ) in combination with IAA (0.5 mg l -l ). The in vitro raised shoots (>3cm long) were excised aseptically and implanted on MS half and full strength medium fortified with auxins (IAA, NAA and IBA) at the concentrations of 0.5, 1.0 and 2.0 mg l -l for root formation. Maximum per cent roots induction and length was obtained on MS full strength medium supplemented with 1.0 mg l -l IAA. Seventy per cent plantlets were successfully established in earthen pots containing soil and sand mixture (3:1). The plantlets were then transferred to the field conditions. The regenerated plants were morphologically uniform and exhibited similar growth characteristics.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 3
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot