Title: Efficient plantlet regeneration from nodal explant culture of Blackgram (Vigna mungo L.) Hepper
Abstract: Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1 was cultured on MS medium containing various concentrations of cytokinins BAP/Kn/TDZ (0.5-3.0 mg/L) alone and also in combination with auxins IAA (0.5 mg/L). Maximum number of shoot bud proliferation was observed at (0.5 mg/L) IAA + (2.0 mg/L).TDZ, compared to all other concentrations of Kn/BAP alone. As the concentration was increased above (2.0mg/L) the shoot bud induction was reduced gradually in both the cytokinins tested. The synergistic effect of both auxin and cytokinin combination was found to be effective in inducing maximum number of shoots. High frequency of shoots was induced at (0.5mg/L IAA + (2.5mg/L) Kn and (0.5mg/L) IAA+ (2.0mg/L) TDZ. The in vitro regenerated shoots produced more number of roots on MS medium containing (1.0mg/L) IBA. Thus the plant developed in vitro using nodal cultures were established in pots containing garden soil outside under shade in wound temperature and light conditions. These plants flowered after 8 weeks following transfer to pots. The protocol established can be used for rapid multiplication of the specific producing true to type plants.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 2
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