Title: Prevalent location of flat dysplastic aberrant crypt foci near lymphoid follicles in the colon of azoxymethane-treated rats.
Abstract: In the colon of F344 rats treated with 2 x 15 mg/kg body weight of azoxymethane (AOM), the density (number of lesions/cm 2 /rat) of flat aberrant crypt foci (ACF) was 13-fold higher (p<0.05) in the surface area of mucosa immediately adjacent to lymphoid follicles compared with the density of these lesions in the rest of the mucosa. A similar prevalent location near lymphoid follicles was observed for tumours, but not for the classic elevated ACF. The lymphoid follicle- associated flat ACF had the same characteristics as those located in the rest of the mucosa: i.e. severe dysplasia and Wnt pathway stimulation. Aberrant crypt foci (ACF) are described as putative preneoplastic lesions in the colon of carcinogen-treated rodents (1) and in human colorectal cancer. Because ACF protrude towards the lumen, they are easily scored by light microscopic surface examination of formalin-fixed whole- mount colon preparations stained with methylene blue, and they are frequently used as a short-term bioassay to evaluate the role of nutritional components and chemopreventive agents at an early stage of colon carcinogenesis. Recently, flat ACF with severe dysplasia were discovered, in addition
Publication Year: 2006
Publication Date: 2006-05-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 3
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