A Comprehensive Structure-Function Map of the Intracellular Surface of the Human C5a Receptor

Name: Work Video:
Duration: 3 min 30 s
M Matsumoto; Kirk Narzinski; Gregory V. Nikiforovich; Thomas Baranski
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975, 1005, 1018, 1056, 1320, 1410, 1659, 1665, 1697, 1799, 1862, 1878, 1895, 1967, 1978, 2023, 2032, 2040, 2059, 2091, 2209, 2215, 2225, 2378, 2382, 2395, 2487, 2509, 2522, 2544, 2636, 2645, 2654, 2761, 2798, 2857, 2883, 2930, 2938, 2984, 2990, 3046, 3139, 3159, 3172, 3176, 3226, 3234, 3269, 3282, 3376, 3381, 3402, 3412, 3439, 3442, 3451, 3481, 3495, 3499, 3508, 3555, 3568, 3573, 3591, 3605, 3616, 3647, 3654, 3708, 3727, 3745, 3748, 3753, 3790, 3799, 3808, 3831, 3837, 3857, 3875, 3911, 3918, 3942, 3995, 4015, 4027, 4049, 4084, 4096, 4118, 4153, 4165, 4187, 4225, 4237, 4259, 4307, 4316, 4349, 4371, 4374, 4417, 4429, 4432, 4459, 4470, 4485], 'multiple': [14, 275, 2068], 'proteins,': [16, 277, 621], 'yet': [17, 278], 'most': [18, 171, 279, 432, 1299, 1321], 'couple': [20, 281, 951, 1022, 1076, 3999, 4323, 4381, 4464, 4489], 'to': [21, 28, 108, 121, 132, 148, 188, 239, 251, 258, 282, 289, 369, 382, 393, 409, 449, 500, 512, 519, 660, 670, 673, 676, 734, 764, 778, 794, 796, 800, 845, 947, 952, 962, 998, 1021, 1023, 1039, 1077, 1313, 1507, 1644, 1793, 1885, 2012, 2025, 2049, 2067, 2088, 2095, 2499, 2517, 2547, 2585, 2628, 2765, 2808, 2969, 3428, 3551, 3561, 3645, 3711, 3849, 3877, 3932, 3998, 4000, 4310, 4322, 4324, 4364, 4380, 4382, 4411, 4422, 4449, 4465, 4491, 4503], 'a': [22, 47, 64, 76, 124, 184, 211, 226, 241, 283, 308, 325, 337, 385, 445, 472, 487, 502, 566, 744, 754, 933, 960, 973, 979, 1044, 1314, 1660, 1965, 2045, 2083, 2207, 2240, 2376, 2409, 2492, 2531, 2548, 2560, 2579, 2596, 2680, 2685, 2796, 2850, 2884, 2916, 2964, 2975, 3054, 3437, 3563, 3574, 3579, 3595, 3623, 3655, 3696, 3730, 3750, 4002, 4008, 4360, 4397, 4442, 4494], 'specific': [23, 284], 'subset': [24, 285, 974], 'proteins': [27, 257, 288, 518, 740, 941, 946, 2094], 'elicit': [29, 290], 'their': [30, 291, 732, 797, 1033, 3426, 3847], 'programmed': [31, 292], 'response.': [32, 293, 804, 967], 'Numerous': [33, 294], 'studies': [34, 295], 'demonstrate': [35, 296, 1083, 3906], 'that': [36, 176, 248, 297, 437, 509, 613, 697, 842, 932, 989, 1075, 1084, 2015, 2030, 2064, 2074, 2086, 2428, 3641, 3962, 4335, 4358, 4377, 4436, 4441, 4463, 4468, 4488], 'carboxyl-terminal': [38, 150, 163, 231, 299, 411, 424, 492, 1797, 1898, 2034], 'five': [39, 151, 300, 412, 1875, 2020, 3505], 'amino': [40, 152, 301, 413, 1680, 1693, 1876, 2021, 2213, 2379, 2393, 2986, 3496, 3506, 4046, 4064, 4115, 4133, 4184, 4202, 4256, 4274, 4392], 'acids': [41, 153, 302, 414, 1694, 1877, 2022, 2214, 2380, 2394, 3497, 3507], 'Gα': [44, 142, 305, 403, 775, 788, 832, 976, 1079, 1699, 1880, 2024, 2070, 2218, 2385, 3904, 3937, 4011], 'subunits': [45, 143, 306, 404, 816, 833, 906, 912, 917, 977, 3905, 3938], 'major': [48, 309], 'determinant': [49, 174, 310, 435], 'specificity,': [51, 312], 'however': [52, 313], 'receptor': [54, 73, 168, 315, 334, 429, 995, 1045, 1672, 1788, 3044, 3121, 3553, 3617, 3642, 3697, 3758, 3869], 'determinants': [55, 316, 3554], 'specificity': [57, 111, 173, 242, 318, 372, 434, 503, 980, 1059, 1509, 1903, 2084, 3556, 3815, 3909], 'less': [59, 320], 'clear.': [60, 321], 'We': [61, 322, 1962, 2028], 'have': [62, 323, 1501, 1673, 1869, 1882, 1963, 2114, 3637], 'used': [63, 324, 528, 1964, 2497, 2584, 3052, 3633], 'collection': [65, 326, 1966, 3926], '133': [67, 328, 1968, 3416, 3920, 3960], 'functional': [68, 329, 1969, 3417, 3924, 4051, 4120, 4189, 4261], 'mutants': [69, 179, 330, 440, 1971, 2035, 2043, 3421, 3483, 3961, 3997, 4017, 4086, 4155, 4227, 4301, 4318, 4334, 4357], 'C5a': [72, 167, 333, 428, 607, 994, 2244, 2413, 3146, 3839, 3944], 'obtained': [74, 335, 1972, 3359, 4054, 4123, 4192, 4264], 'mutagenesis': [77, 338, 1506, 1976, 2554, 4032, 4101, 4170, 4242], 'screen': [78, 339], 'targeting': [79, 340], 'intracellular': [81, 202, 237, 342, 463, 498, 579, 583, 587, 1034, 1070, 1291, 1657, 1980, 2080, 3141, 3418, 4297, 4352], 'loops': [82, 238, 343, 499, 1292, 1658, 2081, 3142, 3167], 'and': [83, 98, 344, 359, 736, 747, 780, 789, 813, 846, 851, 860, 922, 942, 1000, 1157, 1294, 1671, 1676, 1689, 1908, 2036, 2061, 2078, 2100, 2111, 2373, 2400, 2421, 2507, 2528, 2578, 2613, 2620, 2663, 2679, 2790, 2811, 2842, 2862, 2908, 2972, 3009, 3062, 3069, 3114, 3164, 3215, 3285, 3354, 3386, 3390, 3613, 3769, 3827, 3863, 3952, 4021, 4057, 4069, 4090, 4126, 4138, 4159, 4195, 4207, 4231, 4267, 4279, 4304, 4415], 'carboxyl': [85, 346, 546, 553, 560, 1306, 2076], 'terminus': [86, 347, 1307, 1681, 2077], '(Matsumoto,': [87, 348], 'M.': [88, 349, 888, 1132, 1134, 1443, 1840, 1940, 2129, 3529, 3672], 'L.,': [89, 350], 'Narzinski,': [90, 351, 3002], 'K.,': [91, 352], 'Kiser,': [92, 353, 3005], 'P.': [93, 354, 1479, 1725, 1727, 2154, 2252, 2435, 2700, 3003], 'D.,': [94, 355], 'Nikiforovich,': [95, 356, 3008], 'G.': [96, 357, 3006], 'V.,': [97, 358], 'Baranski,': [99, 360, 3012], 'T.': [100, 361, 1138, 1383, 2164, 2262, 2445, 2710, 3010], 'J.': [101, 103, 362, 364, 884, 1088, 1090, 1091, 1108, 1113, 1141, 1160, 1162, 1163, 1201, 1217, 1234, 1241, 1255, 1274, 1275, 1324, 1326, 1327, 1345, 1362, 1384, 1419, 1420, 1444, 1463, 1465, 1477, 1513, 1515, 1516, 1533, 1539, 1541, 1562, 1575, 1579, 1580, 1600, 1626, 1627, 1807, 1825, 1843, 1996, 2160, 2169, 2258, 2267, 2441, 2450, 2706, 2715, 2741, 3011, 3188, 3251, 3308, 3464], '(2007)': [102, 363], 'Biol.': [104, 365, 872, 1092, 1114, 1142, 1164, 1242, 1256, 1276, 1328, 1346, 1385, 1421, 1445, 1517, 1581, 1628, 1808, 1826, 1844, 1997, 2170, 2268, 2294, 2451, 2477, 2716, 2742, 2831, 3093, 3189, 3252, 3309, 3465], 'Chem.': [105, 366, 1093, 1115, 1143, 1165, 1243, 1257, 1277, 1329, 1347, 1386, 1422, 1446, 1518, 1582, 1629, 1809, 1827, 1845, 1998, 2171, 2269, 2452, 2717, 2743, 3190, 3253, 3310, 3466], '282,': [106, 367], '3105–3121)': [107, 368], 'investigate': [109, 370, 1508], 'how': [110, 371], 'is': [112, 169, 373, 430, 692, 1029, 3619, 3628, 4035, 4104, 4173, 4245, 4447, 4480, 4500], 'encoded.': [113, 374], 'Each': [114, 375, 968, 3917], 'mutant,': [115, 376], 'originally': [116, 377, 3423, 3964], 'selected': [117, 378, 3424, 3965], 'for': [118, 216, 244, 379, 477, 505, 653, 731, 959, 982, 2922, 2950, 2956, 3133, 3154, 3209, 3223, 3273, 3291, 3325, 3328, 3331, 3334, 3337, 3340, 3343, 3346, 3349, 3352, 3356, 3363, 3388, 3397, 3487, 3582, 3609, 3761, 3843, 3929, 3966, 4031, 4100, 4169, 4241], 'its': [119, 380], 'ability': [120, 381, 672, 733, 1017, 3427, 3560, 3707, 3848, 3931, 3994, 4321], 'signal': [122, 383, 795, 844, 918, 1047, 3429, 3933, 4344, 4412, 4423], 'through': [123, 384, 919, 971, 1048, 2501, 2915, 3934, 4338, 4413, 4424, 4452], 'nearly': [125, 177, 386, 438, 688], 'full-length': [126, 387], 'Gαi': [127, 388, 911, 999, 3801, 3883], 'yeast,': [129, 390], 'was': [130, 391, 2232, 2538, 2559, 2583, 2763, 2814, 2933, 3051, 3276, 3759, 3817, 3989], 'tested': [131, 392, 3486, 3760, 3928], 'see': [133, 394], 'whether': [134, 395], 'it': [135, 396, 1037, 3704], 'could': [136, 397], 'activate': [137, 398, 737, 963], 'three': [138, 399, 2643, 3490, 4416], 'versions': [139, 400], 'chimeric': [141, 402, 1065, 2069, 3903, 3936], 'consisting': [144, 405], 'Gpa1': [146, 407, 3500], 'fused': [147, 408], 'Gαi,': [155, 416, 4019, 4088, 4157, 4229], 'Gαq,': [156, 417, 2398, 4020, 4089, 4158, 4230, 4414], 'or': [157, 418, 2415, 3515, 3657, 3716, 3736, 3811, 3892, 4007, 4355], 'Gαs': [158, 419, 905, 2052, 3516, 3812, 3893, 4022, 4091, 4160, 4232, 4326, 4425], 'yeast.': [160, 421], 'Surprisingly': [161, 422], 'tail': [164, 232, 425, 493, 1798], 'important': [172, 433, 1300], 'all': [178, 439, 689, 704, 1656, 2031, 2495, 3398], 'this': [181, 217, 442, 478, 784, 3635, 4478, 4498], 'region': [182, 443, 1981, 3175, 4029, 4098, 4167, 4239], 'showed': [183, 444, 3886, 4359], 'gain': [185, 446, 2046, 4361, 4398], 'coupling': [187, 448, 1318, 2048, 2066, 4363, 4439], 'Gαq': [189, 450, 916, 1007, 1011, 2050, 2855, 3018, 3024, 3513, 3810, 3891, 4312, 4340, 4366, 4384, 4453, 4466, 4505], 'and/or': [190, 451, 1407, 2051], 'Gαs.': [191, 452, 1014], 'More': [192, 453], 'than': [193, 454, 625, 2038], 'half': [194, 455, 550, 557, 2039], 'mutated': [198, 459], 'second': [201, 462, 556, 1086, 4351], 'loop': [203, 464, 580, 584, 588, 1071, 1496, 1686, 3405, 3419, 4298, 4353], 'also': [204, 465, 1502, 1790, 4420], 'demonstrated': [205, 466, 1792, 2044, 3781, 3870], 'broadened': [206, 218, 467, 479, 4438], 'protein': [208, 246, 469, 507, 1058, 1670, 1867, 1892, 1902, 2504, 2941, 3566, 3611, 3626, 3702], 'coupling.': [209, 470, 4400], 'Given': [210, 471, 931], 'lack': [212, 473, 2777], 'selective': [214, 475], 'advantage': [215, 476], 'signaling': [219, 480, 803, 2500, 2634, 2770, 3488, 3643, 3725, 3762, 3844, 3871, 3888, 3967, 4337, 4451], 'initial': [222, 483], 'screen,': [223, 484], 'we': [224, 485, 990, 2072], 'propose': [225, 486, 2073], 'model': [227, 488], 'which': [229, 490, 948, 1041, 2693, 3435], 'acts': [233, 494, 2087], 'together': [234, 495], 'with': [235, 496, 565, 1405, 1795, 2017, 2239, 2407, 2670, 2793, 2795, 2854, 2899, 2935, 2974, 3023, 3109, 3124, 3369, 3787, 3881, 3889, 4040, 4067, 4109, 4136, 4178, 4205, 4250, 4277, 4396], 'generate': [240, 501], 'filter': [243, 504, 2085], 'receptor-G': [245, 506, 1866], 'interactions': [247, 508], 'functions': [249, 510], 'primarily': [250, 511], 'restrict': [252, 513, 2089], 'access': [253, 514, 2090], 'incorrect': [255, 516, 2092], 'receptor.': [260, 521, 608, 984, 1662, 2097], '(GPCRs)': [525], '2The': [526], 'abbreviations': [527], 'are:': [529], 'GPCR,': [530], 'receptor;': [533, 544], '3AT,': [534, 3749], '3-aminotriazole;': [535], 'C5a,': [536], 'complement': [537, 541], 'factor': [538, 542], '5a;': [539], 'C5aR,': [540], '5a': [543], 'CT,': [545], 'terminus;': [547, 554, 561], 'CT1,': [548], 'first': [549], 'CT2,': [555], 'CT2stop323,': [562], 'truncated': [563], 'C5aR': [564, 1970, 2427, 2489, 2546, 2592, 2672, 2683, 2769, 2779, 2800, 3106, 3177, 3420, 3757, 3780, 3792, 3833, 3913, 3976], 'stop': [567, 2549, 2686, 4214, 4286], 'codon': [568, 2542, 2550, 2687], 'at': [569, 2608, 2925, 2947, 2959, 4497], 'position': [570, 4499], '323;': [571], 'Endo-Hf,': [572], 'endo-β-N-acetylglucosaminidase': [573, 2939], 'H;': [574], 'ER,': [575], 'endoplasmic': [576], 'reticulum;': [577], 'IC1,': [578], '1;': [581], 'IC2,': [582], '2;': [585], 'IC3,': [586, 1408], '3;': [589], 'IP3,': [590], 'inositol': [591], '1,4,5-triphosphate;': [592], 'r.m.s.,': [593], 'root': [594], 'mean': [595], 'square': [596], 'deviation;': [597], 'RSM,': [598], 'random': [599, 1974], 'saturation': [600, 1975], 'mutagenesis;': [601], 'W5Cha,': [602], 'hexapeptide': [603, 2801, 3793], 'agonist': [604, 2802, 3794], 'seven': [610], 'transmembrane-spanning': [611], 'constitute': [614], 'one': [615], 'largest': [618], 'families': [619], 'encoded': [622], 'by': [623, 694, 752, 761, 836, 1068, 1505, 1973, 2222, 2234, 2389, 2540, 2570, 2606, 2641, 2786, 2868, 2913, 3035, 3231, 3238, 3372, 3425, 3448, 3502, 3773, 3819, 3845], 'more': [624, 2037, 4003, 4009], '1%': [626], 'human': [628, 2227, 2396, 3452, 3509, 3576, 3597, 4004], 'genes': [629], '(1Miller': [630, 708], 'K.J.': [631, 709], 'Murphy': [632, 710], 'B.J.': [633, 711], 'Pelleymounter': [634, 712], 'M.A.': [635, 713, 1236, 1938, 1950, 2127, 2139, 3527, 3539, 3670, 3682], 'Curr.': [636, 714], 'Drug': [637, 715], 'Targets': [638, 716], 'CNS': [639, 717], 'Neurol.': [640, 718], 'Disord.': [641, 719], '2004;': [642, 720], '3:': [643, 721, 874], '357-377Crossref': [644, 722], 'PubMed': [645, 723, 826, 876, 900, 1102, 1124, 1152, 1174, 1195, 1211, 1250, 1266, 1286, 1338, 1356, 1395, 1431, 1453, 1469, 1490, 1527, 1552, 1567, 1591, 1605, 1638, 1717, 1740, 1761, 1782, 1816, 1834, 1854, 1924, 1957, 2007, 2146, 2180, 2278, 2298, 2461, 2481, 2726, 2752, 2835, 3097, 3199, 3262, 3319, 3475, 3546, 3689], 'Scopus': [646, 724, 827, 877, 901, 1103, 1125, 1153, 1175, 1196, 1212, 1267, 1287, 1339, 1357, 1396, 1432, 1470, 1491, 1528, 1553, 1568, 1592, 1606, 1639, 1718, 1741, 1762, 1783, 1855, 1925, 1958, 2008, 2147, 2181, 2279, 2299, 2462, 2482, 2727, 2753, 2836, 3098, 3200, 3263, 3320, 3476, 3547, 3690], '(15)': [647, 725], 'Google': [648, 726, 829, 879, 903, 1105, 1127, 1155, 1177, 1198, 1214, 1229, 1251, 1269, 1289, 1341, 1359, 1372, 1398, 1434, 1454, 1472, 1493, 1530, 1555, 1570, 1594, 1608, 1641, 1720, 1743, 1764, 1785, 1817, 1835, 1857, 1927, 1960, 2010, 2149, 2183, 2281, 2301, 2464, 2484, 2729, 2755, 2838, 3100, 3202, 3265, 3322, 3478, 3549, 3692], 'Scholar).': [649, 727, 830, 904, 1494, 1786, 1858, 1961, 2184, 2302, 2485, 2756, 2839, 3203, 3266, 3479, 3693], 'GPCRs': [650, 686, 728, 1066], 'necessary': [652], 'transmission': [654], 'signals': [656, 970], 'across': [657], 'membranes': [659], 'orchestrate': [661], 'essential': [662], 'processes': [663, 691], 'ranging': [664], 'from': [665, 774, 786, 2491, 2562, 2674, 2805], 'fusion': [667, 2208, 2377, 2942], 'yeast': [669, 1909, 2217, 2384, 2516, 2991, 3432, 3444, 3593, 3631, 3710, 3738, 4455], 'mobilize': [674], 'leukocytes': [675], 'sites': [677], 'infection': [679], 'humans.': [681], 'The': [682, 831, 1054, 1787, 2229, 2535, 2556, 3040, 3358, 3559, 3755, 3778, 3867, 4024, 4045, 4093, 4114, 4162, 4183, 4234, 4255], 'central': [683], 'role': [684, 1889], 'physiologic': [690], 'underscored': [693], 'fact': [696], 'they': [698, 843, 849], 'targets': [700, 799], '∼30%': [702], 'available': [705], 'pharmaceutical': [706], 'drugs': [707], 'named': [730], 'bind': [735, 779], 'heterotrimeric': [738], 'made': [741, 2569], 'up': [742, 3876], 'Gα,': [745, 810, 3445], 'Gβ,': [746, 812], 'Gγ': [748, 815, 1801], 'subunit.': [749], 'Activation': [750, 2486], 'occurs': [751], 'transmitting': [753], 'conformational': [755], 'change': [756, 1315], 'receptor,': [759], 'triggered': [760], 'ligand': [762, 3840, 3945], 'binding,': [763, 3612], 'protein.': [767], 'This': [768, 3814], 'catalyzes': [769], 'release': [771], 'GDP': [773], 'allowing': [776, 2514], 'GTP': [777], 'trigger': [781], 'dissociation': [782], 'subunit': [785, 1004, 1700, 1802, 1881, 4012], 'Gβγ.': [787], 'Gβγ': [790], 'then': [792, 3485], 'free': [793], 'downstream': [798, 840, 966], 'begin': [801], 'In': [805, 2524, 3587], 'humans': [806], 'there': [807, 1028, 3988], '17': [809], '5': [811, 2392, 2923, 2957], '12': [814], '(2Wettschureck': [817], 'N.': [818, 1511, 1537, 1806, 1824, 1842], 'Offermanns': [819], 'S.': [820, 1130, 1232, 1381, 1547, 1756, 1777], 'Physiol.': [821], 'Rev.': [822, 869, 896], '2005;': [823, 1487, 2295, 2478, 2832, 3094], '85:': [824], '1159-1204Crossref': [825], '(868)': [828], 'classified': [835], 'type': [838], 'effectors': [841], 'responses': [848], 'evoke': [850], 'can': [852, 1413, 3599, 3733, 3739], 'divided': [853], 'into': [854, 2589, 2849, 2865, 3032, 3066, 3072], 'four': [855], 'families:': [856], 'Gαs,': [857, 2399, 2401], 'Gαi/o,': [858], 'Gαq/11,': [859], 'Gα12/13': [861, 923], '(3Pierce': [862], 'K.L.': [863], 'Premont': [864], 'R.T.': [865], 'Lefkowitz': [866, 1189, 1239, 1440], 'R.J.': [867, 1190, 1240, 1441], 'Nat.': [868, 2291, 2474, 2828, 3090], 'Mol.': [870, 1224, 1367, 2293, 2476, 2830, 3092], 'Cell.': [871], '2002;': [873], '639-650Crossref': [875], '(2163)': [878], 'Scholar,': [880, 1106, 1128, 1178, 1199, 1215, 1230, 1252, 1270, 1342, 1360, 1373, 1435, 1455, 1473, 1531, 1556, 1571, 1595, 1609, 1721, 1744, 1765, 1818, 1836, 1928, 2150, 2282, 2465, 2730], '4Cabrera-Vera': [881], 'T.M.': [882], 'Vanhauwe': [883], 'Thomas': [885], 'T.O.': [886], 'Medkova': [887], 'Preininger': [889], 'A.': [890, 1379, 1548, 1597, 1706, 1757, 1778, 2732], 'Mazzoni': [891], 'M.R.': [892, 1203, 1223, 1366], 'Hamm': [893], 'H.E.': [894, 1702], 'Endocr.': [895], '2003;': [897], '24:': [898], '765-781Crossref': [899], '(524)': [902], 'stimulate': [907], 'adenylyl': [908, 914], 'cyclase,': [909], 'whereas': [910, 4314, 4477], 'inhibit': [913], 'cyclase.': [915], 'phospholipase': [920], 'C,': [921], 'activates': [924, 3698], 'various': [925, 1499], 'Rho': [926], 'guanine': [927], 'nucleotide': [928], 'exchange': [929], 'factors.': [930], 'single': [934, 1661, 3564, 3575, 3596, 3624, 4443], 'may': [936], 'express': [937, 3562, 3717], 'different': [939, 1078, 3364, 3491], 'GPCRs,': [943], 'must': [953], 'be': [954, 1414, 2766, 3600, 3734, 3740], 'tightly': [955], 'controlled': [956], 'order': [958], 'GPCR': [961, 969, 988, 3577, 3598], 'correct': [965], 'only': [972, 1872, 3622, 4305], 'creating': [978], 'profile': [981, 3816, 3910], 'For': [985, 3057], 'example,': [986], 'study,': [991], 'complement-derived': [993], '(C5aR),': [996], 'couples': [997], 'promiscuous': [1002], 'Gα16': [1003, 3058, 3063], 'family,': [1008], 'but': [1009, 3803, 3885], 'not': [1010, 1311, 3804, 4343, 4387, 4481, 4501], 'itself,': [1012], 'nor': [1013], 'Despite': [1015], 'same': [1025], 'protein,': [1027, 2219, 2386], 'little': [1030], 'homology': [1031], 'among': [1032], 'loops,': [1035], 'making': [1036], 'difficult': [1038], 'predict': [1040], 'protein(s)': [1043], 'will': [1046], 'based': [1049, 2771, 3277], 'upon': [1050], 'primary': [1051], 'sequence': [1052, 2594, 4026, 4095, 4164, 4236], 'alone.': [1053], 'nature': [1055], 'has': [1060, 1651, 1789, 2187, 2304, 2321, 2339, 2354], 'been': [1061, 1503, 1652, 1674, 1791, 1870, 1883, 2115, 3638], 'previously': [1062, 2116, 2247, 2430, 2695, 2818, 2996, 3080], 'investigated': [1063], 'using': [1064, 2551, 3955], 'created': [1067, 2233], 'swapping': [1069], 'regions': [1072, 1497], 'between': [1073, 1667], 'subunits.': [1080], 'These': [1081, 2403], 'experiments': [1082], '(5Liu': [1087, 1159, 1323], 'Wess': [1089, 1161, 1273, 1325, 1418, 1514, 1540, 1561, 1578, 1625], '1996;': [1094, 1166, 1330], '271:': [1095, 1167, 1331], '8772-8778Abstract': [1096, 1168, 1332], 'Full': [1097, 1099, 1119, 1121, 1147, 1149, 1169, 1171, 1247, 1261, 1263, 1281, 1283, 1333, 1335, 1351, 1353, 1390, 1392, 1426, 1428, 1450, 1522, 1524, 1586, 1588, 1633, 1635, 1813, 1831, 1849, 1851, 2002, 2004, 2175, 2177, 2273, 2275, 2456, 2458, 2721, 2723, 2747, 2749, 3194, 3196, 3257, 3259, 3314, 3316, 3470, 3472], 'Text': [1098, 1100, 1120, 1122, 1148, 1150, 1170, 1172, 1248, 1262, 1264, 1282, 1284, 1334, 1336, 1352, 1354, 1391, 1393, 1427, 1429, 1451, 1523, 1525, 1587, 1589, 1634, 1636, 1814, 1832, 1850, 1852, 2003, 2005, 2176, 2178, 2274, 2276, 2457, 2459, 2722, 2724, 2748, 2750, 3195, 3197, 3258, 3260, 3315, 3317, 3471, 3473], 'PDF': [1101, 1123, 1151, 1173, 1249, 1265, 1285, 1337, 1355, 1394, 1430, 1452, 1526, 1590, 1637, 1815, 1833, 1853, 2006, 2179, 2277, 2460, 2725, 2751, 3198, 3261, 3318, 3474], '(119)': [1104, 1176, 1340], '6Nasman': [1107], 'Jansson': [1109], 'C.C.': [1110], 'Akerman': [1111], 'K.E.': [1112], '1997;': [1116, 1144, 1258, 1348, 1564, 1583, 1737], '272:': [1117, 1145, 1259, 1349, 1584], '9703-9708Abstract': [1118], '(37)': [1126], '7Verrall': [1129], 'Ishii': [1131], 'Chen': [1133], 'Wang': [1135], 'L.': [1136, 2999], 'Tram': [1137], 'Coughlin': [1139], 'S.R.': [1140], '6898-6902Abstract': [1146], '(63)': [1154], 'Scholar)': [1156, 1290, 2011, 3101, 3550], 'third': [1158], '8Kobilka': [1179], 'B.K.': [1180], 'Kobilka': [1181], 'T.S.': [1182], 'Daniel': [1183], 'K.': [1184, 1746, 1769, 1989, 2288, 2471, 2825, 3001, 3087, 3181, 3244, 3301, 3457], 'Regan': [1185], 'J.W.': [1186], 'Caron': [1187, 1237, 1438], 'M.G.': [1188, 1238, 1439], 'Science.': [1191, 1713, 1736], '1988;': [1192, 1714], '240:': [1193], '1310-1316Crossref': [1194], '(695)': [1197], '9Wess': [1200], 'Brann': [1202, 1222, 1365], 'Bonner': [1204, 1218, 1363], 'T.I.': [1205, 1219, 1364], 'FEBS': [1206], 'Lett.': [1207], '1989;': [1208], '258:': [1209], '133-136Crossref': [1210], '(96)': [1213], '10Wess': [1216], 'Dorje': [1220], 'F.': [1221], 'Pharmacol.': [1225, 1368], '1990;': [1226, 1369], '38:': [1227, 1370], '517-523PubMed': [1228], '11Cotecchia': [1231], 'Ostrowski': [1233], 'Kjelsberg': [1235], '1992;': [1244], '267:': [1245], '1633-1639Abstract': [1246], '12Olah': [1253, 1343], 'M.E.': [1254, 1344, 1622], '337-344Abstract': [1260, 1350], '(77)': [1268, 1358], '13Erlenbach': [1271], 'I.': [1272, 1417, 1611], '1998;': [1278, 1423], '273:': [1279, 1424], '26549-26558Abstract': [1280, 1425], '(42)': [1288, 1433], '(IC2': [1293], 'IC3)': [1295], 'generally': [1296], 'determining': [1302], 'specificity.': [1303, 1893, 2027, 3586], 'Exchanging': [1304], '(CT)': [1308], 'alone': [1309], 'does': [1310, 4386], 'lead': [1312], 'profiles': [1319], '14Wess': [1361], '872-877PubMed': [1371], '15Takagi': [1374], 'Y.': [1375, 1748, 1767], 'Ninomiya': [1376], 'H.': [1377], 'Sakamoto': [1378], 'Miwa': [1380], 'Masaki': [1382], '1995;': [1387, 1519, 1549, 1846], '270:': [1388, 1520, 1847], '10072-10078Abstract': [1389], '(83)': [1397], 'Scholar);': [1399, 1642], 'however,': [1400, 1643], 'when': [1401, 3785], 'swapped': [1402], 'combination': [1404], 'IC2': [1406, 2042, 3404, 4016, 4034, 4404, 4434, 4460], 'activity': [1409, 3784], 'hybrids': [1412], 'enhanced': [1415], '(13Erlenbach': [1416], '16Liggett': [1436], 'S.B.': [1437], 'Hnatowich': [1442], '1991;': [1447], '266:': [1448], '4816-4821Abstract': [1449], '17Pin': [1456], 'J.P.': [1457], 'Joly': [1458], 'C.': [1459, 1577, 1615, 1618, 1733, 1946, 1948, 2135, 2137, 3535, 3537, 3678, 3680], 'Heinemann': [1460], 'S.F.': [1461], 'Bockaert': [1462], 'EMBO': [1464], '1994;': [1466, 1828], '13:': [1467], '342-348Crossref': [1468], '(168)': [1471], '18Kim': [1474], 'J.M.': [1475, 2284, 2467, 2821, 3083], 'Hwa': [1476], 'Garriga': [1478], 'Reeves': [1480], 'P.J.': [1481], 'RajBhandary': [1482], 'U.L.': [1483], 'Khorana': [1484, 1749, 1770], 'H.G.': [1485, 1750, 1771], 'Biochemistry.': [1486, 1563], '44:': [1488], '2284-2292Crossref': [1489], '(135)': [1492], 'Individual': [1495], 'targeted': [1504, 4030, 4099, 4168, 4240], '(19Blin': [1510], 'Yun': [1512, 1538], '17741-17748Abstract': [1521], '(110)': [1529], '20Liu': [1532], 'Conklin': [1534, 1559], 'B.R.': [1535, 1560, 1911], 'Blin': [1536], 'Proc.': [1542, 1751, 1772], 'Natl.': [1543, 1752, 1773], 'Acad.': [1544, 1753, 1774], 'Sci.': [1545, 1754, 1775], 'U.': [1546, 1755, 1776], '92:': [1550], '11642-11646Crossref': [1551], '(199)': [1554], '21Kostenis': [1557], 'E.': [1558, 1573, 1613], '36:': [1565], '1487-1495Crossref': [1566], '(98)': [1569], '22Kostenis': [1572], 'Gomeza': [1574], 'Lerche': [1576], '23675-23681Abstract': [1585], '(69)': [1593], '23Malmberg': [1596], 'Strange': [1598], 'P.G.': [1599], 'Neurochem.': [1601], '2000;': [1602, 1954, 2143, 2744, 3543, 3686], '75:': [1603], '1283-1293Crossref': [1604], '(53)': [1607, 2754], '24Erlenbach': [1610], 'Kostenis': [1612], 'Schmidt': [1614], 'Serradeil-Le': [1616], 'Gal': [1617], 'Raufaste': [1619], 'D.': [1620, 1704, 1917, 3004], 'Dumont': [1621], 'Pausch': [1623], 'M.H.': [1624], '2001;': [1630, 1758, 1779], '276:': [1631], '29382-29392Abstract': [1632], '(55)': [1640], 'our': [1645, 3923], 'knowledge,': [1646], 'no': [1647, 3887, 3990], 'comprehensive': [1648], 'mutational': [1649], 'study': [1650, 3636], 'carried': [1653, 2815], 'out': [1654, 2816], 'on': [1655, 1864, 2055, 2768, 2772, 2963, 3168, 3278, 3713, 3851], 'Specific': [1663], 'points': [1664, 1861], 'contact': [1666, 1863], 'identified,': [1675, 1871], 'these': [1677, 1896, 3482, 4308, 4418, 4471], 'include': [1678], '(residues': [1682, 1687, 1695, 3384, 3406], '8–23),': [1683], 'α4–β6': [1685], '311–328),': [1688], 'last': [1691, 1874, 2019, 2391, 3504], '11': [1692], '340–450)': [1696], '(25Hamm': [1701], 'Deretic': [1703], 'Arendt': [1705], 'Hargrave': [1707], 'P.A.': [1708], 'Koenig': [1709], 'B.': [1710], 'Hofmann': [1711], 'K.P.': [1712], '241:': [1715], '832-835Crossref': [1716], '(421)': [1719], '26Onrust': [1722], 'R.': [1723], 'Herzmark': [1724, 2153, 2251, 2434, 2699], 'Chi': [1726], 'Garcia': [1728], 'P.D.': [1729, 1991, 3183, 3246, 3303, 3459], 'Lichtarge': [1730, 2155, 2253, 2436, 2701, 2735], 'O.': [1731, 1804, 1838, 2156, 2254, 2437, 2702, 2736], 'Kingsley': [1732], 'Bourne': [1734, 1918, 2167, 2265, 2448, 2713], 'H.R.': [1735, 1919, 2168, 2266, 2449, 2714], '275:': [1738, 2745], '381-384Crossref': [1739], '(198)': [1742], '27Cai': [1745], 'Itoh': [1747], '98:': [1759, 1780], '4877-4882Crossref': [1760], '(138)': [1763], '28Itoh': [1766], 'Cai': [1768], '4883-4887Crossref': [1781], '(104)': [1784], 'interact': [1794, 2016], '(29Kisselev': [1803], 'Gautam': [1805, 1823, 1841], '1993;': [1810, 1921], '268:': [1811], '24519-24522Abstract': [1812], '30Kisselev': [1819], 'O.G.': [1820], 'Ermolaeva': [1821, 1839], 'M.V.': [1822], '269:': [1829], '21399-21402Abstract': [1830], '31Kisselev': [1837], '25356-25358Abstract': [1848], '(107)': [1856], 'Although': [1859], 'several': [1860, 4508], 'interface': [1868], 'shown': [1884], 'play': [1886], 'an': [1887, 2416, 2422, 2675], 'integral': [1888], 'Exchange': [1894], 'extreme': [1897], 'residues': [1899, 2014, 2223, 2982, 3377, 3440, 3449], 'confers': [1900, 3705], 'both': [1905], 'mammalian': [1906, 2599, 3915], 'cells': [1907, 2867, 3034, 3103, 3115], '(32Conklin': [1910], 'Farfel': [1912], 'Z.': [1913], 'Lustig': [1914], 'K.D.': [1915], 'Julius': [1916], 'Nature.': [1920], '363:': [1922], '274-276Crossref': [1923], '(626)': [1926], '33Brown': [1929], 'A.J.': [1930, 2119, 3519, 3662], 'Dyos': [1931, 2120, 3520, 3663], 'S.L.': [1932, 2121, 3521, 3664], 'Whiteway': [1933, 2122, 3522, 3665], 'M.S.': [1934, 2123, 3523, 3666], 'White': [1935, 2124, 3524, 3667], 'J.H.': [1936, 2125, 3525, 3668], 'Watson': [1937, 2126, 3526, 3669], 'Marzioch': [1939, 2128, 3528, 3671], 'Clare': [1941, 2130, 3530, 3673], 'J.J.': [1942, 2131, 3531, 3674], 'Cousens': [1943, 2132, 3532, 3675], 'D.J.': [1944, 2133, 3533, 3676], 'Paddon': [1945, 2134, 3534, 3677], 'Plumpton': [1947, 2136, 3536, 3679], 'Romanos': [1949, 2138, 3538, 3681], 'Dowell': [1951, 2140, 3540, 3683], 'S.J.': [1952, 2141, 3541, 3684], 'Yeast.': [1953, 2142, 3542, 3685], '16:': [1955, 2144, 3544, 3687], '11-22Crossref': [1956, 2145, 3545, 3688], '(152)': [1959, 2148, 3548, 3691], '(RSM)': [1977], 'each': [1979, 2646, 2931, 3370], '(Matsumoto': [1982], 'et': [1983, 3295], 'al.,': [1984, 3296], 'Ref.': [1985, 3297], '67Matsumoto': [1986, 3298], 'M.L.': [1987, 3179, 3242, 3299, 3455], 'Narzinski': [1988, 2287, 2470, 2824, 3086, 3180, 3243, 3300, 3456], 'Kiser': [1990, 3182, 3245, 3302, 3458], 'Nikiforovich': [1992, 3184, 3247, 3304, 3460], 'G.V.': [1993, 3185, 3248, 3305, 3461], 'Baranski': [1994, 2289, 2472, 2739, 2826, 3088, 3186, 3249, 3306, 3462], 'T.J.': [1995, 2152, 2250, 2290, 2433, 2473, 2698, 2740, 2827, 3089, 3187, 3250, 3307, 3463], '2007;': [1999, 3191, 3254, 3311, 3467], '282:': [2000, 3192, 3255, 3312, 3468], '3105-3121Abstract': [2001, 3193, 3256, 3313, 3469], '(16)': [2009, 3201, 3264, 3321, 3477], 'identify': [2013], 'confer': [2026], 'found': [2029], 'chimeras.': [2053, 3813, 4023, 4092, 4161, 4233], 'Based': [2054], 'wide': [2057], 'variety': [2058], 'mutations': [2060, 2603], 'truncations': [2062], 'observed': [2063, 4482], 'allowed': [2065], 'subunits,': [2071], 'create': [2082], 'Yeast': [2098, 2618], 'Strains': [2099], 'Plasmids—Yeast': [2101], 'strains': [2102, 2404, 2496, 3492, 3632, 3772, 3947], 'BY1142': [2103, 2186, 2205, 2237], '(Gαi3),': [2104, 2106, 3766, 3824, 3949], 'BY1173': [2105, 2303, 3765, 3823, 3948, 3979], 'BY1172': [2107, 2320, 3767, 3825, 3950], '(Gαq),': [2108, 3768, 3826, 3951], 'BY1401': [2109, 2338, 3828, 3953], '(Gαs),': [2110], 'BY1404': [2112, 2353, 2374, 2529, 2791, 3770], '(Gαs)': [2113, 3771, 3829, 3954], 'described': [2117, 2248, 2431, 2696, 2819, 3081, 3152], '(33Brown': [2118, 3518, 3661], '34Baranski': [2151], 'Gerber': [2157, 2255, 2438, 2703], 'B.O.': [2158, 2256, 2439, 2704], 'Trueheart': [2159, 2257, 2440, 2705], 'Meng': [2161, 2259, 2442, 2707], 'E.C.': [2162, 2260, 2443, 2708], 'Iiri': [2163, 2261, 2444, 2709], 'Sheikh': [2165, 2263, 2446, 2711], 'S.P.': [2166, 2264, 2447, 2712], '1999;': [2172, 2270, 2453, 2718], '274:': [2173, 2271, 2454, 2719], '15757-15765Abstract': [2174, 2272, 2455, 2720], '(122)': [2182, 2280, 2463, 2728], 'Briefly,': [2185], 'genotype': [2189, 2306, 2323, 2341, 2356], 'MATα': [2190], 'far1Δ1442': [2191], 'tbt1-1': [2192], 'fus1Δ::PFUS1-HIS3': [2193, 2315, 2333, 2365], 'can1': [2194, 2312, 2329, 2347, 2362], 'ste14::trp1::LYS2': [2195], 'ste3Δ1156': [2196], 'gpa1': [2197], '(41)-Gαi3': [2198], 'lys2': [2199], 'ura3': [2200, 2308, 2325, 2343, 2358], 'leu2': [2201, 2309, 2326, 2344, 2359], 'trp1': [2202, 2310, 2327, 2345, 2360], 'his3': [2203, 2311, 2328, 2346, 2361], 'ade2.': [2204], 'contains': [2206, 3436], 'amino-terminal': [2211], '41': [2212], 'Gpa1,': [2220, 2387, 3446], 'followed': [2221, 2388, 3237, 3447, 3501], '34–354': [2224, 3450], 'Gαi3.': [2228], 'BY1143': [2230, 3969], 'strain': [2231, 2238, 3433, 3980], 'transforming': [2235], 'URA3': [2241, 2410, 2418], 'plasmid': [2242, 2411, 2424, 2493, 2558], 'encoding': [2243, 2574], '(pBN444)': [2245, 2414], 'as': [2246, 2817, 2995, 3053, 3079, 3150, 3220, 3222, 3971, 3973, 4071, 4140, 4209, 4281, 4345, 4347], '(34Baranski': [2249, 2432, 2697], '35Klco': [2283, 2466], 'Wiegand': [2285, 2468, 2822, 3084], 'C.B.': [2286, 2469, 2823, 3085], 'Struct.': [2292, 2475, 2829, 3091], '12:': [2296, 2479, 2833, 3095], '320-326Crossref': [2297, 2480, 2834, 3096], '(139)': [2300, 2483, 2837, 3099], 'MATa': [2307, 2324, 2342, 2357], 'gpa1Δ::ade2Δ::3XHA': [2313, 2348, 2363], 'far1Δ::ura3Δ': [2314, 2332, 2349, 2364], 'LEU2::PFUS1-lacZ': [2316, 2334, 2366], 'sst2Δ::ura3Δ': [2317, 2335, 2367], 'ste2Δ::G418R': [2318, 2336], 'trp1::GPA1/Gαi3.': [2319], 'gpa1Δ::': [2330], 'ade2Δ::3XHA': [2331], 'trp1::GPA1/Gαq.': [2337], 'fus1Δ::PFUS1-HIS': [2350], 'sst2Δ::ura': [2351], 'Δtrp1::GPA1/Gαs.': [2352], 'ste2ΔG418R': [2368], 'lys2ΔΔtrp1::GPA1/Gαs.': [2369], 'BY1173,': [2370, 2526, 2788], 'BY1172,': [2371, 2527, 2789], 'BY1401,': [2372], 'contain': [2375, 2530, 4473], '1–467': [2381, 3498], 'Gαi3,': [2397], 'respectively.': [2402], 'were': [2405, 2429, 2568, 2604, 2625, 2639, 2668, 2694, 2784, 2847, 2874, 2920, 2954, 2993, 3029, 3064, 3077, 3107, 3122, 3148, 3229, 3367, 3422, 3484, 3841, 3927, 3963, 4302, 4409, 4419], 'transformed': [2406, 2792], 'either': [2408, 4001], 'expressing': [2412, 2425, 3104, 3116], 'empty': [2417], 'vector': [2419, 2852, 3028], '(pBN443)': [2420], 'ADE2': [2423, 2676, 2780], 'leads': [2498, 3644], 'mitogen-activated': [2503], 'kinase': [2505], 'cascade': [2506], 'expression': [2508, 2600, 3653], 'PFUS1-HIS3': [2511, 3656], 'reporter': [2512, 2533, 3659], 'gene,': [2513], 'grow': [2518, 3712, 3850], 'absence': [2521, 2760, 3604], 'histidine.': [2523], 'addition,': [2525], 'PFUS1-β-galactosidase': [2532, 3658], 'gene.': [2534], 'CT2stop323': [2536], 'truncation': [2537], 'generated': [2539], 'mutating': [2541], '323': [2543], 'Pfu': [2552], 'turbo': [2553], '(Stratagene).': [2555], 'RGS4': [2557], 'gift': [2561], 'Dr.': [2563], 'Maurine': [2564], 'Linder.': [2565], 'Mutants': [2566], 'M1–M4': [2567], 'designing': [2571], 'complementary': [2572], 'oligonucleotides': [2573], 'desired': [2576], 'mutation(s),': [2577], 'two-step': [2580], 'PCR': [2581], 'strategy': [2582], 'introduce': [2586], 'mutation(s)': [2588], 'wild-type': [2591, 2671, 3756, 3779, 3832, 3868, 3975, 4025, 4094, 4163, 4235], 'coding': [2593], 'pcDNA3.1(+)': [2597, 3067], '(Invitrogen)': [2598, 3068], 'vector.': [2601], 'All': [2602, 3204, 3480, 3630, 3959], 'verified': [2605], 'sequencing': [2607], 'Washington': [2610], 'University': [2611], 'Protein': [2612, 3410], 'Nucleic': [2614], 'Acid': [2615], 'Chemistry': [2616], 'Laboratory.': [2617], 'Transformation': [2619], 'Receptor': [2621], 'Signaling': [2622, 2666], 'Assays—Yeast': [2623], 'transformations': [2624], 'done': [2626, 2785, 2994], 'according': [2627], 'standard': [2629, 2869, 3036], 'lithium': [2630], 'acetate': [2631], 'protocols.': [2632], 'Relative': [2633], 'abilities': [2635], 'mutant': [2637, 2647, 2682, 3145, 3365, 3728, 3925, 4052, 4121, 4190, 4262, 4375, 4405, 4433, 4461], 'assayed': [2640, 3842], 'restreaking': [2642], 'transformants': [2644, 3830], 'onto': [2648], 'histidine-deficient': [2649, 3714, 3852], 'medium': [2650, 3715, 3853], 'containing': [2651, 2684], 'varying': [2652], 'amounts': [2653, 3747, 3789], '3-amino-1,2,4-triazole': [2655], '(3AT)': [2656], '(Sigma)': [2657], '(0,': [2658], '1,': [2659, 3859], '5,': [2660, 3860, 3899, 3985], '10,': [2661, 3861], '20,': [2662, 3862], '50': [2664, 2926, 2960, 3342, 3864, 3878], 'mm).': [2665], 'levels': [2667, 3076], 'compared': [2669, 3368, 4066, 4135, 4204, 4276], 'plasmid,': [2677], 'pBN482,': [2678], 'non-functional': [2681], 'transmembrane': [2689, 3174, 3382], 'helix': [2690], '3,': [2691, 3897, 3983], 'pBN483,': [2692], '36Geva': [2731], 'Lassere': [2733], 'T.B.': [2734], 'Pollitt': [2737], 'S.K.': [2738], '35393-35401Abstract': [2746], 'Growth': [2757], 'histidine': [2762], 'inferred': [2764], 'dependent': [2767], 'colony': [2773], 'color': [2774], '(red': [2775], 'colonies': [2776], 'plasmid).': [2781], 'β-Galactosidase': [2782], 'assays': [2783], 'treating': [2787], 'pBN482': [2794], 'range': [2797], 'W5Cha': [2803, 3112, 3795], '(GenScript)': [2804, 3113], '10-10': [2806], 'm': [2807, 3126], '10-5': [2809], 'm,': [2810], 'assay': [2813, 3732, 3775], '(35Klco': [2820, 3082], 'Endo-β-N-acetylglucosaminidase': [2840], 'Treatment': [2841], 'Western': [2843, 2988], 'Blots': [2844], '—RSM': [2845], 'subcloned': [2848, 3065], 'pIRES': [2851, 3027], '(Clontech)': [2853], '(University': [2856, 3045], 'Missouri-Rolla': [2858, 3047], 'cDNA': [2859, 3048], 'Resource': [2860, 3049], 'Center)': [2861, 3050], 'transiently': [2863, 3030, 3070], 'transfected': [2864, 3031], 'HEK293': [2866, 3033, 3073], 'calcium': [2870, 3037], 'phosphate': [2871, 3038], 'methods.': [2872, 3039], 'Cells': [2873], 'lysed': [2875], '2': [2876], 'days': [2877], 'after': [2878], 'transfection': [2879], '250': [2881], 'μl': [2882, 2929], '1': [2885, 2902, 2905, 3110, 4299], '×': [2886], 'sample': [2887], 'buffer': [2888], '(50': [2889], 'mm': [2890, 3865, 3879], 'Tris-Cl,': [2891], 'pH': [2892], '6.8,': [2893], '2%': [2894, 2900], 'SDS,': [2895], '10%': [2896], 'glycerol)': [2897], 'supplemented': [2898], 'β-mercaptoethanol,': [2901], 'μg/ml': [2903, 2906], 'leupeptin,': [2904], 'aprotinin,': [2907], '500': [2909], 'μm': [2910, 3111], 'phenylmethylsulfonyl': [2911], 'fluoride': [2912], 'shearing': [2914], '27G1/2': [2917], 'syringe.': [2918], 'Lysates': [2919], 'heated': [2921, 2955], 'min': [2924, 2958], '°C.': [2927], '27': [2928, 3327], 'lysate': [2932], 'treated': [2934, 3108, 3123, 3786], '1000': [2936], 'units': [2937], 'H-maltose-binding': [2940], '(Endo-Hf,': [2943], 'New': [2944], 'England': [2945], 'Biolabs)': [2946], '37': [2948], '°C': [2949], '3': [2951], 'h.': [2952], 'Samples': [2953], '°C,': [2961], 'resolved': [2962], '12%': [2965], 'SDS-PAGE': [2966], 'gel,': [2967], 'transferred': [2968], 'polyvinylidene': [2970], 'difluoride,': [2971], 'immunoblotted': [2973], 'rabbit': [2976], 'polyclonal': [2977], 'anti-C5aR': [2978], 'antibody': [2979], 'raised': [2980], 'against': [2981], '9–29': [2983], 'terminus.': [2987], 'blots': [2989], 'lysates': [2992], 'described.': [2997], '3M.': [2998], 'Matsumoto,': [3000], 'V.': [3007], 'unpublished': [3013], 'observation.': [3014], 'Inositol': [3015], '1,4,5-Triphosphate': [3016], 'Accumulation—For': [3017], 'signaling,': [3019, 3059], 'RSM': [3021, 3060], 'm1': [3041, 3118], 'muscarinic': [3042, 3119], 'acetylcholine': [3043, 3120], 'positive': [3055, 4495], 'control.': [3056], 'co-transfected': [3071], 'cells.': [3074, 3916], 'IP3': [3075], 'measured': [3078], 'where': [3102], '10-4': [3125], 'carbachol': [3127], '(Sigma).': [3128], 'Molecular': [3129], 'Modeling—Molecular': [3130], 'modeling': [3131], 'procedures': [3132], 'restoring': [3134], 'low': [3135, 3206, 3270, 3288, 3360], 'energy': [3136, 3207, 3239, 3271, 3280, 3289, 3361], 'backbone': [3137, 3400], 'conformations': [3138, 3208, 3272], 'exactly': [3149], 'those': [3151], 'earlier': [3153], 'WT,': [3156], 'including': [3157], 'employment': [3158], 'ECEPP/2': [3161], 'force': [3162], 'field,': [3163], 'mounting': [3165], 'three-dimensional': [3170], 'structure': [3171], '(67Matsumoto': [3178, 3241, 3454], 'possible': [3205], 'IC1': [3210, 4333], '(fragment': [3211], '63–71),': [3212], 'IC2-(138–150),': [3213], 'IC3-(224–236),': [3214], 'C-terminal': [3217], 'fragment': [3218], '300–310,': [3219], 'well': [3221, 3972], '“package”': [3225], 'IC1+IC2+IC3+fragment': [3227], '300–310': [3228], 'determined': [3230], 'subsequent': [3232], 'application': [3233], 'geometrical': [3235], 'sampling': [3236], 'minimization': [3240], 'Final': [3267], 'selection': [3268], 'package': [3275], 'cut-off': [3281], '30': [3283, 4403], 'kcal/mol': [3284], 'yielded': [3286], '56': [3287], 'structures': [3290, 3362], 'WT': [3292], '(see': [3293, 4368], 'Matsumoto': [3294], 'Scholar),': [3323], '22': [3324], 'R35,': [3326], 'R91,': [3329], '33': [3330], 'R89,': [3332], '35': [3333], 'R40,': [3335], '28': [3336, 3355], 'R58,': [3338], '44': [3339], 'R111,': [3341], 'R52,': [3344], '45': [3345], 'M1,': [3347], '47': [3348], 'M2,': [3350], '46': [3351], 'M3,': [3353], 'M4.': [3357], 'other': [3371, 3606], 'overlapping': [3373], 'spatial': [3374], 'positions': [3375], 'comprising': [3378], 'stems': [3380], 'helices': [3383], '135–138': [3385], '150–153': [3387], 'IC2)': [3389], 'calculating': [3391], 'so-called': [3393], '“global”': [3394], 'r.m.s.': [3395], 'values': [3396], 'heavy': [3399], 'atoms': [3401], '139–149).': [3407], 'Determining': [3408], 'Coupling': [3411], 'Intracellular': [3413], 'Loop': [3414], 'Mutants—The': [3415], 'BY1143,': [3434], 'chimera': [3438, 3567, 3627, 3802, 3884, 4327, 4341, 4367, 4385], '1–41': [3441], 'Gαi3': [3453, 3510], 'carrying': [3493], 'chimeras': [3494, 3894], '(strain': [3511], 'BY1173),': [3512], '(BY1172),': [3514], '(BY1401)': [3517], 'elucidate': [3552], '(Fig.': [3557, 3776, 4328, 4426, 4456], '1a).': [3558], 'interest': [3569], 'provides': [3578], 'powerful': [3580], 'tool': [3581], 'studying': [3583], 'protein-coupling': [3585], 'isolated': [3589], 'environment': [3590], 'cell,': [3594], 'studied': [3601], 'competing': [3608], 'readout': [3615], 'activation': [3618, 3646], 'clear,': [3620], 'because': [3621, 4507], 'expressed.': [3629], 'engineered': [3639], 'so': [3640], 'yeast-mating': [3649], 'pathway': [3650], 'resulting': [3651], 'gene': [3660], 'Thus,': [3694, 3901, 3987], 'if': [3695], 'particular': [3700], 'expressed,': [3703], 'β-galactosidase': [3719, 3731, 3774, 3783], 'enzyme.': [3720], 'To': [3721], 'quantify': [3722], 'relative': [3724], 'strength': [3726], 'performed': [3735], 'grown': [3741], 'increasing': [3746, 3788], 'competitive': [3751], 'inhibitor': [3752], 'His3.': [3754], '1b).': [3777], 'dose-dependent': [3782], 'confirmed': [3818], 'growth': [3821, 3957], 'assay.': [3822, 3958], 'testing': [3846], '0,': [3858], '3AT.': [3866], '3AT': [3880], '(Figs.': [3895, 3981], '2,': [3896, 3982], '4,': [3898, 3984], '6).': [3900, 3986], 'natural': [3908], 'signaled': [3970], 'difference': [3991], 'Gαi3-like': [4005], '(BY1143)': [4006], 'Gpa1-like': [4010], '(BY1173).FIGURE': [4013], '3Signaling': [4014], 'via': [4018, 4087, 4156, 4228], '(top': [4037, 4106, 4175, 4247], 'bold)': [4039, 4108, 4177, 4249], 'residue': [4042, 4111, 4180, 4252], 'numbers': [4043, 4112, 4181, 4253], 'marked.': [4044, 4113, 4182, 4254], 'acid': [4047, 4065, 4116, 4134, 4185, 4203, 4257, 4275, 4393], 'sequences': [4048, 4117, 4186, 4258, 4373, 4431, 4462], '(designated': [4055, 4124, 4193, 4265], 'R': [4056, 4125, 4194, 4266], 'numbered,': [4058, 4127, 4196, 4268], 'left)': [4059, 4128, 4197, 4269], 'indicated': [4061, 4130, 4199, 4271], '(dots,': [4062, 4131, 4200, 4272], 'unchanged': [4063, 4132, 4201, 4273], 'wild-type)': [4068, 4137, 4206, 4278], 'annotated': [4070, 4139, 4208, 4280], 'Fig.': [4073, 4142, 4211, 4283], '2.View': [4074, 4143], 'Large': [4075, 4144, 4216, 4288], 'Image': [4076, 4145, 4217, 4289], 'Figure': [4077, 4146, 4218, 4290], 'ViewerDownload': [4078, 4147, 4219, 4291], 'Hi-res': [4079, 4148, 4220, 4292], 'image': [4080, 4149, 4221, 4293], 'Download': [4081, 4150, 4222, 4294], '(PPT)FIGURE': [4082, 4151, 4223], '4Signaling': [4083], 'IC3': [4085, 4103], '5Signaling': [4152], 'CT1': [4154, 4172], '2.': [4212, 4284], '@,': [4213, 4285], 'codon.View': [4215, 4287], '6Signaling': [4224], 'CT2': [4226, 4244], '(PPT)': [4295], 'Twenty-eight': [4296], '(IC1)': [4300], 'tested,': [4303, 4407], 'nine': [4306, 4469], 'coupled': [4309], 'chimera,': [4313], 'none': [4315], 'gained': [4319], '2).': [4329], 'Of': [4330, 4401], 'note,': [4331], 'show': [4336, 4437, 4467], 'do': [4342], 'strongly': [4346], '(IC2)': [4354], 'CT': [4356], 'below).': [4369], 'Comparison': [4370, 4458], 'able': [4379, 4410, 4421], 'reveal': [4388], 'trend': [4390], 'substitutions': [4394], 'associated': [4395], '20': [4408], '3).': [4427, 4457], 'Analysis': [4428], 'reveals': [4440], 'point': [4444], 'mutation,': [4445, 4476], 'Q145R,': [4446], 'sufficient': [4448, 4502], 'allow': [4450, 4504], 'Q145R': [4475], 'mutation': [4479], 'exclusively': [4490], 'Gαi.': [4492], 'Interestingly,': [4493], 'charge': [4496], 'coupling,': [4506], 'recepto': [4509]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2170672524', 'counts_by_year': [{'year': 2021, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 1}], 'updated_date': '2024-12-12T05:08:45.728312', 'created_date': '2016-06-24'}
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