Publication Information

{'id': 'https://openalex.org/W2934153478', 'doi': 'https://doi.org/10.1111/bcpt.13228', 'title': 'Live cell optical assay for precise characterization of receptors coupling to Gα12', 'display_name': 'Live cell optical assay for precise characterization of receptors coupling to Gα12', 'publication_year': 2019, 'publication_date': '2019-03-28', 'ids': {'openalex': 'https://openalex.org/W2934153478', 'doi': 'https://doi.org/10.1111/bcpt.13228', 'mag': '2934153478', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/30916867', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/6764917'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1111/bcpt.13228', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S44074210', 'display_name': 'Basic & Clinical Pharmacology & Toxicology', 'issn_l': '1742-7835', 'issn': ['1742-7835', '1742-7843'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764917', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5004786447', 'display_name': 'Ikuo Masuho', 'orcid': 'https://orcid.org/0000-0001-8754-987X'}, 'institutions': [{'id': 'https://openalex.org/I123431417', 'display_name': 'Scripps Research Institute', 'ror': 'https://ror.org/02dxx6824', 'country_code': 'US', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I123431417']}], 'countries': ['US'], 'is_corresponding': True, 'raw_author_name': 'Ikuo Masuho', 'raw_affiliation_strings': ['Department of Neuroscience The Scripps Research Institute Florida Jupiter Florida'], 'affiliations': [{'raw_affiliation_string': 'Department of Neuroscience The Scripps Research Institute Florida Jupiter Florida', 'institution_ids': ['https://openalex.org/I123431417']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5035333896', 'display_name': 'Nickolas K. Skamangas', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I123431417', 'display_name': 'Scripps Research Institute', 'ror': 'https://ror.org/02dxx6824', 'country_code': 'US', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I123431417']}], 'countries': ['US'], 'is_corresponding': True, 'raw_author_name': 'Nickolas K. Skamangas', 'raw_affiliation_strings': ['Department of Neuroscience The Scripps Research Institute Florida Jupiter Florida'], 'affiliations': [{'raw_affiliation_string': 'Department of Neuroscience The Scripps Research Institute Florida Jupiter Florida', 'institution_ids': ['https://openalex.org/I123431417']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5061375714', 'display_name': 'Kirill A. Martemyanov', 'orcid': 'https://orcid.org/0000-0002-9925-7599'}, 'institutions': [{'id': 'https://openalex.org/I123431417', 'display_name': 'Scripps Research Institute', 'ror': 'https://ror.org/02dxx6824', 'country_code': 'US', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I123431417']}], 'countries': ['US'], 'is_corresponding': True, 'raw_author_name': 'Kirill A. Martemyanov', 'raw_affiliation_strings': ['Department of Neuroscience The Scripps Research Institute Florida Jupiter Florida'], 'affiliations': [{'raw_affiliation_string': 'Department of Neuroscience The Scripps Research Institute Florida Jupiter Florida', 'institution_ids': ['https://openalex.org/I123431417']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': ['https://openalex.org/A5004786447', 'https://openalex.org/A5035333896', 'https://openalex.org/A5061375714'], 'corresponding_institution_ids': ['https://openalex.org/I123431417', 'https://openalex.org/I123431417', 'https://openalex.org/I123431417'], 'apc_list': {'value': 4220, 'currency': 'USD', 'value_usd': 4220, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 0.164, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 4, 'citation_normalized_percentile': {'value': 0.536677, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 77, 'max': 79}, 'biblio': {'volume': '126', 'issue': 'S6', 'first_page': '88', 'last_page': '95'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11178', 'display_name': 'Receptor Mechanisms and Signaling', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11178', 'display_name': 'Receptor Mechanisms and Signaling', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10814', 'display_name': 'Neuropeptides and Animal Physiology', 'score': 0.9984, 'subfield': {'id': 'https://openalex.org/subfields/2804', 'display_name': 'Cellular and Molecular Neuroscience'}, 'field': {'id': 'https://openalex.org/fields/28', 'display_name': 'Neuroscience'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10169', 'display_name': 'Protein Kinase Regulation and GTPase Signaling', 'score': 0.9975, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/gq-alpha-subunit', 'display_name': 'Gq alpha subunit', 'score': 0.6219751}, {'id': 'https://openalex.org/keywords/hek-293-cells', 'display_name': 'HEK 293 cells', 'score': 0.51206666}, {'id': 'https://openalex.org/keywords/gi-alpha-subunit', 'display_name': 'Gi alpha subunit', 'score': 0.47543883}], 'concepts': [{'id': 'https://openalex.org/C135285700', 'wikidata': 'https://www.wikidata.org/wiki/Q38173', 'display_name': 'G protein-coupled receptor', 'level': 3, 'score': 0.95787466}, {'id': 'https://openalex.org/C139407321', 'wikidata': 'https://www.wikidata.org/wiki/Q408561', 'display_name': 'Heterotrimeric G protein', 'level': 4, 'score': 0.91828656}, {'id': 'https://openalex.org/C80631254', 'wikidata': 'https://www.wikidata.org/wiki/Q407438', 'display_name': 'G protein', 'level': 3, 'score': 0.7849891}, {'id': 'https://openalex.org/C176303035', 'wikidata': 'https://www.wikidata.org/wiki/Q5513929', 'display_name': 'Gs alpha subunit', 'level': 4, 'score': 0.67677826}, {'id': 'https://openalex.org/C66135807', 'wikidata': 'https://www.wikidata.org/wiki/Q24726231', 'display_name': 'Gq alpha subunit', 'level': 4, 'score': 0.6219751}, {'id': 'https://openalex.org/C170493617', 'wikidata': 'https://www.wikidata.org/wiki/Q208467', 'display_name': 'Receptor', 'level': 2, 'score': 0.6188016}, {'id': 'https://openalex.org/C174510640', 'wikidata': 'https://www.wikidata.org/wiki/Q489618', 'display_name': 'HEK 293 cells', 'level': 3, 'score': 0.51206666}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.47674286}, {'id': 'https://openalex.org/C161473406', 'wikidata': 'https://www.wikidata.org/wiki/Q24719569', 'display_name': 'Gi alpha subunit', 'level': 4, 'score': 0.47543883}, {'id': 'https://openalex.org/C96305047', 'wikidata': 'https://www.wikidata.org/wiki/Q367473', 'display_name': 'Förster resonance energy transfer', 'level': 3, 'score': 0.44382972}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.43734354}, {'id': 'https://openalex.org/C104292427', 'wikidata': 'https://www.wikidata.org/wiki/Q899781', 'display_name': 'Protein subunit', 'level': 3, 'score': 0.42786404}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.38159758}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.34580374}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.16068485}, {'id': 'https://openalex.org/C91881484', 'wikidata': 'https://www.wikidata.org/wiki/Q191807', 'display_name': 'Fluorescence', 'level': 2, 'score': 0.0781067}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C62520636', 'wikidata': 'https://www.wikidata.org/wiki/Q944', 'display_name': 'Quantum mechanics', 'level': 1, 'score': 0.0}], 'mesh': [], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1111/bcpt.13228', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S44074210', 'display_name': 'Basic & Clinical Pharmacology & Toxicology', 'issn_l': '1742-7835', 'issn': ['1742-7835', '1742-7843'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764917', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'acceptedVersion', 'is_accepted': True, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764917', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'acceptedVersion', 'is_accepted': True, 'is_published': False}, 'sustainable_development_goals': [{'score': 0.8, 'display_name': 'Affordable and clean energy', 'id': 'https://metadata.un.org/sdg/7'}], 'grants': [{'funder': 'https://openalex.org/F4320332161', 'funder_display_name': 'National Institutes of Health', 'award_id': 'DA036596'}], 'datasets': [], 'versions': [], 'referenced_works_count': 37, 'referenced_works': ['https://openalex.org/W1788638640', 'https://openalex.org/W1818335498', 'https://openalex.org/W1980725116', 'https://openalex.org/W1982641394', 'https://openalex.org/W1996794995', 'https://openalex.org/W2002199803', 'https://openalex.org/W2006031720', 'https://openalex.org/W2011232105', 'https://openalex.org/W2018812790', 'https://openalex.org/W2027764322', 'https://openalex.org/W2032187164', 'https://openalex.org/W2041658762', 'https://openalex.org/W2056913859', 'https://openalex.org/W2060345532', 'https://openalex.org/W2064483656', 'https://openalex.org/W2068244483', 'https://openalex.org/W2069584773', 'https://openalex.org/W2075209136', 'https://openalex.org/W2075366797', 'https://openalex.org/W2077421316', 'https://openalex.org/W2079858948', 'https://openalex.org/W2085801794', 'https://openalex.org/W2091238879', 'https://openalex.org/W2109014958', 'https://openalex.org/W2111478744', 'https://openalex.org/W2145196374', 'https://openalex.org/W2148310842', 'https://openalex.org/W2153283433', 'https://openalex.org/W2165130241', 'https://openalex.org/W2184364461', 'https://openalex.org/W2418027364', 'https://openalex.org/W2765153686', 'https://openalex.org/W2765484602', 'https://openalex.org/W2803649094', 'https://openalex.org/W2809064859', 'https://openalex.org/W2896064918', 'https://openalex.org/W4239945442'], 'related_works': ['https://openalex.org/W4292869918', 'https://openalex.org/W4252004600', 'https://openalex.org/W2329963186', 'https://openalex.org/W2059147358', 'https://openalex.org/W2017663972', 'https://openalex.org/W1991491265', 'https://openalex.org/W1977171037', 'https://openalex.org/W1967487813', 'https://openalex.org/W1522965874', 'https://openalex.org/W125358232'], 'abstract_inverted_index': {'Abstract': [0], 'Heterotrimeric': [1], 'G': [2, 8, 22, 43, 176, 232], 'proteins': [3], 'are': [4], 'essential': [5], 'mediators': [6], 'of': [7, 21, 60, 79, 99, 111, 122, 169, 175, 200, 210, 216, 226], 'protein‐coupled': [9], 'receptors': [10], '(GPCRs)': [11], 'signalling': [12, 190], 'to': [13, 76, 86, 107, 153, 157, 163, 172, 203, 223], 'intracellular': [14], 'effectors.': [15], 'There': [16], 'is': [17, 92, 128, 221], 'a': [18], 'considerable': [19, 66], 'diversity': [20], 'protein': [23, 44, 177], 'subunits': [24, 46, 91, 125], 'that': [25, 149], 'channel': [26], 'signals': [27], 'initiated': [28], 'by': [29, 81, 206], 'GPCRs': [30, 82, 205], 'into': [31], 'specific': [32], 'outcome.': [33], 'In': [34, 116], 'particular,': [35], 'mammalian': [36], 'genomes': [37], 'contain': [38], '16': [39], 'conserved': [40], 'genes': [41], 'encoding': [42], 'α': [45], 'with': [47, 83], 'unique': [48], 'properties.': [49], 'Of': [50], 'four': [51], 'Gα': [52, 193], 'subfamilies': [53], '(Gi/o,': [54], 'Gq,': [55], 'Gs': [56], 'and': [57, 147, 159, 179], 'G12/13),': [58], 'members': [59], 'the': [61, 84, 89, 100, 150, 167, 198, 217], 'G12/13': [62, 80], 'group': [63], 'have': [64], 'received': [65], 'attention': [67], 'for': [68], 'their': [69], 'roles': [70], 'in': [71, 113, 130, 155], 'carcinogenesis.': [72], 'However,': [73], 'our': [74], 'ability': [75], 'study': [77], 'activation': [78, 178], 'power': [85], 'distinguish': [87], 'between': [88], 'two': [90], 'limited.': [93], 'Here,': [94], 'we': [95, 141], 'present': [96], 'an': [97], 'adaptation': [98], 'bioluminescence': [101], 'resonance': [102], 'energy': [103], 'transfer': [104], '(BRET)‐based': [105], 'assay': [106, 171, 202, 220], 'specifically': [108], 'monitor': [109], 'activity': [110], 'Gα12': [112, 127, 154, 208], 'living': [114], 'cells.': [115], 'this': [117, 139, 170, 201, 230], 'kinetic': [118], 'assay,': [119, 140], 'agonist‐induced': [120], 'release': [121], 'Venus‐tagged': [123], 'Gβγ': [124], 'from': [126], 'followed': [129], 'real': [131], 'time': [132], 'using': [133], 'nano‐luciferase': [134], '(Nluc)–tagged': [135], 'BRET': [136, 219], 'donor.': [137], 'Using': [138], 'characterized': [142], 'bradykinin': [143], 'B2': [144], 'receptor': [145, 151, 213], '(BDKRB2)': [146], 'found': [148], 'couples': [152], 'addition': [156], 'Gαo,': [158], 'Gαq,': [160], 'but': [161], 'not': [162], 'Gαs.': [164], 'We': [165, 195], 'demonstrated': [166], 'utility': [168, 199], 'quantify': [173], 'rates': [174], 'inactivation': [180], 'as': [181, 183], 'well': [182], 'performing': [184], 'dose‐response': [185], 'studies': [186], 'while': [187], 'rank': [188], 'ordering': [189], 'via': [191], 'individual': [192], 'subunits.': [194], 'further': [196], 'showed': [197], 'other': [204], 'demonstrating': [207], 'coupling': [209], 'cholecystokinin': [211], 'A': [212], '(CCKAR).': [214], 'Introduction': [215], 'Gα12‐coupling': [218], 'expected': [222], 'accelerate': [224], 'characterization': [225], 'GPCR': [227], 'actions': [228], 'on': [229], 'understudied': [231], 'protein.': [233]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2934153478', 'counts_by_year': [{'year': 2023, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 1}], 'updated_date': '2024-12-15T08:02:21.456961', 'created_date': '2019-04-11'}