Title: EFFECT OF GENOTYPES AND GROWTH REGULATORS ON PLANT REGENERATION FROM LEAF EXPLANTS IN TOMATO
Abstract: The seeds of four genotypes of tomato viz., ‘Castle Rock’, ‘Punjab Upma’, ‘VFN-8’ and ‘IPA-3’ were surface sterilized with a commercial bleach for 20 minutes followed by four to five rinses in sterile water and germinated on half strength (MS inorganic salts were reduced to half) Murashige and Skoog (MS, 1962) media containing thiamine HCl (0.1 mg l -1 ), pyridoxine HCl (0.5 mg l -1 ), nicotinic acid (0.5 mg l -1 ), myo-inositol (100 mg l -1 ), sucrose (3.0 g l -1 ) and agar (0.8%). The pH of medium was adjusted to 5.8 by adding 1N NaOH/1N HCl solution drop-wise. The in vitro germinated seedlings served as an explant source for direct plant regeneration studies. Leaves were excised from in vitro grown seedlings and inoculated onto MS medium supplemented with combinations and concentrations of BAP and kinetin (Table 1). The medium was first dispensed into glass jars (100 ml/jar) when still hot. These glass jars were properly capped before autoclaving at 1.05 kg cm -1 square pressure and 121°C temperature for 22 minutes. Analysis of variance (ANOVA) for experimental design is given in Table 1 which revealed the significance of genotypes (g), hormonal concentrations (h) and their interaction for percent plant regeneration and number of shoots per explant. This indicated that in vitro response of leaf explants was genotype dependent and differed significantly with the kind and concentration of the growth regulators. In our experiment, culture media supplemented with BAP alone and in combination with kinetin were used for direct plant regeneration studies omitting callus phase in four genotypes of tomato i.e., Castle Rock, Punjab Upma, VFN-8 and IPA-3. Leaves were excised from one month old seedlings grown in vitro. Plant regeneration occurred directly from the leaf explant in all the genotypes without intervening callus phase. No plant regeneration occurred on the explants when cultured on the basal MS media devoid of growth regulators. With the increase in the concentration of BAP in the nutrient media, there was a significant increase in per plant regeneration in all the genotypes. The maximum plant regeneration was recorded on R 6 media i.e., MS + BAP 2.0 mg l -1 + kinetin 1.0 mg l -1 in all genotypes. The subsequent higher doses of
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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