Title: Submerged liquid fermentation of some Tanzanian basidiomycetes for the production of mycelial biomass, exopolysaccharides and mycelium protein using wastes peels media.
Abstract:Mycelia from five species of higher fungi of genera Ganoderma, Pleurotus and Laetiporus were used in this study. These were cultured for the investigation of higher exopolysaccharides and mycelia biom...Mycelia from five species of higher fungi of genera Ganoderma, Pleurotus and Laetiporus were used in this study. These were cultured for the investigation of higher exopolysaccharides and mycelia biomass production. Mycelial growths of all species were measured on six different non-defined complex media prepared from waste peels and cattail rhizome; Yam dextrose agar (YADA), cassava dextrose agar (CADA), potato malt extract peptone dextrose agar (PMPDA), sweet potato dextrose agar (SPDA), plantain dextrose agar (PLDA) and cattail rhizome dextrose agar (CATDA). The average mycelial growth rate on the aforementioned solid media ranged between 10 ± 2.6 and 14 ±1.7 mm/day with the highest growth rate obtained from YADA followed by PMPDA while the lowest was recorded from PLDA. The best media based on mycelial growth were selected for further investigation. Therefore, the effect of YAD and PMPD complex media on the submerged mycelia growth and exopolysaccharides, mycelium protein production in five mushrooms was investigated in shake-flask culture at an ambient temperature 30 o C±2. The maximum mycelial growth (14g/l) and exopolysaccharides production (540mg/l) were achieved in YAD medium by Pleurotus spp and Pleurotus HK-37, respectively. The crude protein content of mushroom mycelium in YAD medium varied among the mushroom species. The Pleurotus spp mycelium contained 55% crude protein, which was the highest followed by 41% obtained from Pleurotus HK-37 mycelium. The genus Pleurotus amongst the other mushroom genera investigated, should be considered for reasonable production of mycelial biomass, exopolysaccharides and mycelium protein using YAD medium in submerged fermentation. In conclusion, the data obtained in this study provides useful information for further investigation of higher fungi in submerged culture using waste peels complex media.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 24
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