Abstract: YeastVolume 12, Issue 16 p. 1607-1633 Review Pyruvate Metabolism in Saccharomyces cerevisiae JACK T. PRONK, JACK T. PRONK Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The NetherlandsSearch for more papers by this authorH. YDE STEENSMA, H. YDE STEENSMA Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The NetherlandsSearch for more papers by this authorJOHANNES P. VAN DIJKEN, JOHANNES P. VAN DIJKEN Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The NetherlandsSearch for more papers by this author JACK T. PRONK, JACK T. PRONK Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The NetherlandsSearch for more papers by this authorH. YDE STEENSMA, H. YDE STEENSMA Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The NetherlandsSearch for more papers by this authorJOHANNES P. VAN DIJKEN, JOHANNES P. VAN DIJKEN Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The NetherlandsSearch for more papers by this author First published: December 1996 https://doi.org/10.1002/(SICI)1097-0061(199612)12:16<1607::AID-YEA70>3.0.CO;2-4Citations: 533AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract In yeasts, pyruvate is located at a major junction of assimilatory and dissimilatory reactions as well as at the branch-point between respiratory dissimilation of sugars and alcoholic fermentation. This review deals with the enzymology, physiological function and regulation of three key reactions occurring at the pyruvate branch-point in the yeast Saccharomyces cerevisiae: (i) the direct oxidative decarboxylation of pyruvate to acetyl-CoA, catalysed by the pyruvate dehydrogenase complex, (ii) decarboxylation of pyruvate to acetaldehyde, catalysed by pyruvate decarboxylase, and (iii) the anaplerotic carboxylation of pyruvate to oxaloacetate, catalysed by pyruvate carboxylase. Special attention is devoted to physiological studies on S. cerevisiae strains in which structural genes encoding these key enzymes have been inactivated by gene disruption. References 1 Alani, E., Cao, L. and Kleckner, N. 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